Overview

  • Product nameAnti-CX3CR1 antibody
    See all CX3CR1 primary antibodies
  • Description
    Rabbit polyclonal to CX3CR1
  • SpecificityWe have received customer feedback that ab8021 may not be specific for mouse CX3CR1. Because of this we have removed mouse as a guaranteed species. We welcome additional feedback for using this antibody in mouse. Please contact our Scientific Support team for more information.
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to N terminal amino acids 2-21 of Human CX3CR1 (Peptide available as ab8125.)

  • Positive control
    • Human spleen tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab8021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/100.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Can be blocked with CX3CR1 peptide (ab8125).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionReceptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
  • Tissue specificityExpressed in lymphoid and neural tissues.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta chemokine receptor-like 1 antibody
    • C X3 C CKR 1 antibody
    • C-X3-C CKR-1 antibody
    • CCRL1 antibody
    • Chemokine C X3 C motif receptor 1 antibody
    • CMK BRL 1 antibody
    • CMK-BRL-1 antibody
    • CMK-BRL1 antibody
    • CMKBLR1 antibody
    • CMKDR1 antibody
    • CX3C chemokine receptor 1 antibody
    • CX3C CKR1 antibody
    • CX3C1_HUMAN antibody
    • CX3CR1 antibody
    • Fractalkine receptor antibody
    • G-protein coupled receptor 13 antibody
    • GPR13 antibody
    • GPRV28 antibody
    • V28 antibody
    see all

Anti-CX3CR1 antibody images

  • All lanes : Anti-CX3CR1 antibody (ab8021)

    Lane 1 : Human spleen lysate with absence of blocking peptide.
    Lane 2 : Human spleen lysate with presence of blocking peptide.


    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-CX3CR1 at 2 µg/ml.
  • Immunofluorescence of CX3CR1 in Human Heart cells using ab8021 at 10 ug/ml.

  • Flow cytometry analysis of THP-1 cells using CX3CR1 antibody (ab8021) at 0.1 ug/ml. 

    Green: Isotype control

    Red: CX3CR1 antibody (ab8021) 


  • Developed using the ECL technique

    Performed under reducing conditions.

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

References for Anti-CX3CR1 antibody (ab8021)

This product has been referenced in:
  • Ali I  et al. Role of fractalkine-CX3CR1 pathway in seizure-induced microglial activation, neurodegeneration, and neuroblast production in the adult rat brain. Neurobiol Dis 74:194-203 (2015). Read more (PubMed: 25461978) »
  • Ruchaya PJ  et al. The cardiovascular actions of fractalkine/CX3CL1 in the hypothalamic paraventricular nucleus are attenuated in rats with heart failure. Exp Physiol 99:111-22 (2014). Rat . Read more (PubMed: 24036597) »

See all 18 Publications for this product

Product Wall

Thank you for contacting us. The alignment of the ab8021 immunogen sequence with the protein at the following link showed 90% identity, so we expect the antibody will react with your samples.

http://www.ncbi.nlm.nih.gov/protein/355559765?rep...

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Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step None
Sample Rat Tissue sections (Rat spinal cord with lesion)
Specification Rat spinal cord with lesion
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 03 2015

Application Immunocytochemistry
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Human Cultured Cells (human microglia cell induced from monocyte)
Specification human microglia cell induced from monocyte
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 03 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Rat Tissue lysate - whole (Rat spinal cord with lesion on different days)
Specification Rat spinal cord with lesion on different days
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 03 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 8,5
Sample Human Tissue sections (Heart)
Specification Heart
Permeabilization Yes - Wash Buffer from Dako with Tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

Submitted May 28 2014

Application Flow Cytometry
Fixation Paraformaldehyde
Sample Mouse Cell (BM)
Specification BM
Gating Strategy monocyte
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Apr 25 2014

Thank you for contacting us about the issue you are having with the most recent vial. We have not had any complaints about lot GR82315-1 but if all your reagents and samples are the same as what you used for successful stains, then it is possible you r...

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Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

This antibody was tested for flow cytometry in an outside laboratory, and all of the protocol details are not available. I...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. Your enquiry has been forwarded by #### to the scientific support team and we will be pleased to help you in this...

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Thank you for your reply, and I apologize for this inconvenience.

I certainly understand that ab9858 is not as useful to you as ab73680 would be. I can issue a credit to you.

The vial of ab8020 should be arriving shortly, please kee...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"