• Product name
    Anti-CXCL11 antibody [10C6]
    See all CXCL11 primary antibodies
  • Description
    Mouse monoclonal [10C6] to CXCL11
  • Host species
  • Tested applications
    Suitable for: WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant full length Human CXCL11 expressed in E.coli (O14625).

  • Positive control
    • Induced HeLa cell culture supernatant.



Our Abpromise guarantee covers the use of ab155936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 10 kDa.
Sandwich ELISA Use a concentration of 1 - 3 µg/ml.

Use as coating antibody at 1μg/ml and 0.125-0.25 μg/ml for detection.


  • Function
    Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
  • Tissue specificity
    High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • b R1 antibody
    • b-R1 antibody
    • Beta-R1 antibody
    • betaR1 antibody
    • C-X-C motif chemokine 11 antibody
    • Chemokine (C-X-C motif) ligand 11 antibody
    • Chemokine C-X-C motif ligand 11 antibody
    • CXC11 antibody
    • CXCL11 antibody
    • CXL11_HUMAN antibody
    • H174 antibody
    • I TAC antibody
    • I-TAC antibody
    • Interferon gamma inducible protein 9 antibody
    • Interferon gamma-inducible protein 9 antibody
    • Interferon inducible T cell alpha chemoattractant antibody
    • Interferon-inducible T-cell a chemoattractant I-TAC antibody
    • Interferon-inducible T-cell alpha chemoattractant antibody
    • IP 9 antibody
    • IP-9 antibody
    • IP9 antibody
    • ITAC antibody
    • MGC102770 antibody
    • SCYB11 antibody
    • SCYB9B antibody
    • Small inducible cytokine B11 antibody
    • Small inducible cytokine subfamily B (Cys-X-Cys) member 11 antibody
    • Small inducible cytokine subfamily B (Cys-X-Cys) member 9B antibody
    • Small inducible cytokine subfamily B, member 11 antibody
    • Small inducible cytokine subfamily B, member 9B antibody
    • Small-inducible cytokine B11 antibody
    see all


  • All lanes : Anti-CXCL11 antibody [10C6] (ab155936) at 1/1000 dilution

    Lane 1 : Recombinant Human CXCL11
    Lane 2 : Induced HeLa cell culture supernatant

    Lysates/proteins at 0.25 µg per lane.

    All lanes : Goat anti-mouse HRP conjugated antibody at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 10 kDa

    Western blot analysis was performed using a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane, blocked with 5% Milk/TBST for at least 1 and then incubated with ab155936 overnight at 4ºC with rocking. Following washing with TBS/0.1% Tween 20, the membrane was incubated with secondary antibody for at least 1 hour and washed again. Chemiluminescent detection was the performed.
  • Sandwich ELISA analysis of Human CXCL11 was performed using an ELISA Kit by coating a blank 96-well microtiter plate with 100µl per well of ab155936 in duplicate at 1, 3, 4, 5, 7, and 9µg/ml in DPBS and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300µl per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human CXCL11 recombinant protein at 50µl per well was added in duplicate at 4000, 1600, 640, 256, 102.4, 40.96, and 0 pg/ml for 2 hours at room temperature along with 50µl of a Human CXCL11 biotinylated monoclonal antibody in all applicable wells at 0.2µg/ml for 2 hours at room temperature. The plate was washed and incubated with 100µl per well of Streptavidin-HRP in all test wells at 1:80,000 dilution for 1 hour at room temperature and then washed and incubated with 100µl per well of TMB substrate for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.


ab155936 has not yet been referenced specifically in any publications.

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