The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 10 kDa.
Use a concentration of 1 - 3 µg/ml.
Use as coating antibody at 1μg/ml and 0.125-0.25 μg/ml for detection.
Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
Belongs to the intercrine alpha (chemokine CxC) family.
Small inducible cytokine subfamily B (Cys-X-Cys) member 11 antibody
Small inducible cytokine subfamily B (Cys-X-Cys) member 9B antibody
Small inducible cytokine subfamily B, member 11 antibody
Small inducible cytokine subfamily B, member 9B antibody
Small-inducible cytokine B11 antibody
Western blot - Anti-CXCL11 antibody [10C6] (ab155936)
All lanes : Anti-CXCL11 antibody [10C6] (ab155936) at 1/1000 dilution
Lane 1 : Recombinant Human CXCL11 Lane 2 : Induced HeLa cell culture supernatant
Lysates/proteins at 0.25 µg per lane.
Secondary All lanes : Goat anti-mouse HRP conjugated antibody at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 10 kDa
Western blot analysis was performed using a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane, blocked with 5% Milk/TBST for at least 1 and then incubated with ab155936 overnight at 4ºC with rocking. Following washing with TBS/0.1% Tween 20, the membrane was incubated with secondary antibody for at least 1 hour and washed again. Chemiluminescent detection was the performed.
Sandwich ELISA analysis of Human CXCL11 was performed using an ELISA Kit by coating a blank 96-well microtiter plate with 100µl per well of ab155936 in duplicate at 1, 3, 4, 5, 7, and 9µg/ml in DPBS and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300µl per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human CXCL11 recombinant protein at 50µl per well was added in duplicate at 4000, 1600, 640, 256, 102.4, 40.96, and 0 pg/ml for 2 hours at room temperature along with 50µl of a Human CXCL11 biotinylated monoclonal antibody in all applicable wells at 0.2µg/ml for 2 hours at room temperature. The plate was washed and incubated with 100µl per well of Streptavidin-HRP in all test wells at 1:80,000 dilution for 1 hour at room temperature and then washed and incubated with 100µl per well of TMB substrate for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.