Overview

  • Product nameAnti-CXCL14 antibody
    See all CXCL14 primary antibodies
  • Description
    Rabbit polyclonal to CXCL14
  • Tested applicationsELISA, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hBRAK (human BRAK).

  • General notesThe sterile filtered antibody solution was lyophilized from 0.5xPBS, pH7.4.

Properties

  • FormLyophilised:Reconstitute 100µl sterile water to give a concentration 1 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab46010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml. Detection limit 0.2 - 0.4 ng/well.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa. Detection limit 1.5 - 3.0 ng/lane under reducing or non-reducting conditions.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent dilution.

Target

  • FunctionPotent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactive for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
  • Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110031L23Rik antibody
    • 1200006I23Rik antibody
    • AI414372 antibody
    • BMAC antibody
    • bolekine antibody
    • BRAK antibody
    • C-X-C motif chemokine 14 antibody
    • Chaemokine, CXC motif, ligand 14 antibody
    • Chemokine (C-X-C motif) ligand 14 antibody
    • Chemokine BRAK antibody
    • CXC chemokine in breast and kidney antibody
    • Cxcl14 antibody
    • CXL14_HUMAN antibody
    • JSC antibody
    • Kec antibody
    • Kidney-expressed chemokine CXC antibody
    • KS1 antibody
    • MGC10687 antibody
    • MGC124510 antibody
    • MGC90667 antibody
    • MIP 2 gamma antibody
    • MIP-2G antibody
    • MIP2G antibody
    • MIP2gamma antibody
    • NJAC antibody
    • PRO273 antibody
    • PSEC0212 antibody
    • Scyb14 antibody
    • Small Inducible Cytokine B14 antibody
    • Small inducible cytokine subfamily B (Cys-X-Cys) member 14 (BRAK) antibody
    • Small Inducible Cytokine subfamily B, member 14 antibody
    • Small nducible cytokine B14 antibody
    • Small-inducible cytokine B14 antibody
    • UNQ240 antibody
    see all

Anti-CXCL14 antibody images

  • ab46010 staining Cxcl14/BRAK in human breast invasive, ductal carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • ab46010 staining Cxcl14/BRAK in human breast invasive, ductal carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • ICC/IF image of ab46010 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46010 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CXCL14 antibody (ab46010)

This product has been referenced in:
  • Riester M  et al. Risk Prediction for Late-Stage Ovarian Cancer by Meta-analysis of 1525 Patient Samples. J Natl Cancer Inst 106:N/A (2014). IHC-P ; Human . Read more (PubMed: 24700803) »
  • Leach RE  et al. High throughput, cell type-specific analysis of key proteins in human endometrial biopsies of women from fertile and infertile couples. Hum Reprod 27:814-828 (2012). IHC-P ; Human . Read more (PubMed: 22215622) »

See all 3 Publications for this product

Product Wall

Thank you, the images all arrived. I think you are correct, the TIFF files were probably too big.

Have you been able to obtain good stains for other proteins using this protocol?

I think the protocol is correct as it is. A custome...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"