The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes ELISA: Use at a concentration of 0.15 - 0.3 µg/ml. Allows the detection of at least 0.2 ng/well of recombinant human CXCL16.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human CXCL16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 33 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.
Tissue specificityExpressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
Cellular localizationCell membrane. Secreted. Also exists as a soluble form.