Anti-CXCL2 antibody (ab9841)
Key features and details
- Rabbit polyclonal to CXCL2
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-CXCL2 antibody
See all CXCL2 primary antibodies -
Description
Rabbit polyclonal to CXCL2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human CXCL2 aa 1-150.
Database link: P19875 -
Positive control
- WB: Recombinant human CXCL2 protein (ab9842).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.1 - 0.2 µg/ml.
The detection limit for recombinant human CXCL2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
Use 100 µl/well antibody solution. Detects of 0.2 - 0.4 ng/well of recombinant human CXCL2. |
Notes |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human CXCL2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Use 100 µl/well antibody solution. Detects of 0.2 - 0.4 ng/well of recombinant human CXCL2. |
Target
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Function
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsThe N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 2 antibody
- Chemokine (C X C motif) ligand 2 antibody
- Chemokine, CXC motif, ligand 2 antibody
see all
Images
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To detect human CXCL2 by Western Blot analysis ab9841 can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant human CXCL2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is reducing conditions; Human recombinant CXCL2 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant CXCL2 protein.
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To detect human CXCL2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9841 is required. This antigen affinity purified antibody, in conjunction with anti-CXCL2 (Biotin) (ab245843) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human CXCL2 .
Datasheets and documents
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Datasheet download
References (0)
ab9841 has not yet been referenced specifically in any publications.