Overview

  • Product nameAnti-CXCL5 antibody
    See all CXCL5 primary antibodies
  • Description
    Rabbit polyclonal to CXCL5
  • Tested applicationsSuitable for: ELISA, Neutralising, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hENA-78 (Human Epithelial Neutrophil Activating Peptide-78).

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human pancreas adenocarcinoma.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hENA-78 (100.0 ng/ml), a concentration of 15 - 20 µg/ml of this antibody is required.
WB Use a concentration of 0.1 - 0.2 µg/ml.
IP 1/2500.
IHC-P Use a concentration of 1 µg/ml.

Target

  • FunctionInvolved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMCFII antibody
    • C-X-C motif chemokine 5 antibody
    • chemokine (C-X-C motif) ligand 5 antibody
    • Cxcl5 antibody
    • CXCL5_HUMAN antibody
    • ENA-78 (8-78) antibody
    • ENA-78(1-78) antibody
    • ENA-78(9-78) antibody
    • ENA78 antibody
    • Epithelial derived neutrophil activating protein 78 antibody
    • Epithelial-derived neutrophil-activating protein 78 antibody
    • Lipopolysaccharide-induced CXC chemokine antibody
    • Neutrophil activating peptide ENA 78 antibody
    • Neutrophil-activating peptide ENA-78 antibody
    • neutrophil-activating protein 78 antibody
    • SCYB5 antibody
    • Small inducible cytokine B5 antibody
    • small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) antibody
    • small inducible cytokine subfamily B, member 5 antibody
    • Small-inducible cytokine B5 antibody
    see all

Anti-CXCL5 antibody images

  • IHC image of CXCL5 staining in Human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9802, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-CXCL5 antibody (ab9802)

This product has been referenced in:
  • Hsu YL  et al. Breast tumor-associated osteoblast-derived CXCL5 increases cancer progression by ERK/MSK1/Elk-1/Snail signaling pathway. Oncogene : (2012). IP . Read more (PubMed: 23045282) »
  • Wang Q  et al. Mutant proteins as cancer-specific biomarkers. Proc Natl Acad Sci U S A 108:2444-9 (2011). IP ; Human . Read more (PubMed: 21248225) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (human pancreas and colon)
Total protein in input 5000 µg
Specification human pancreas and colon
Immuno-precipitation step Protein G
Username

Mr. Qing Wang

Verified customer

Submitted Aug 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"