The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA:To detect mLIX by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mLIX.
Western Blot:To detect mLIX by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLIX is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
Belongs to the intercrine alpha (chemokine CxC) family.
N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
Epithelial derived neutrophil activating protein 78 antibody
Epithelial-derived neutrophil-activating protein 78 antibody
Lipopolysaccharide-induced CXC chemokine antibody
Neutrophil activating peptide ENA 78 antibody
Neutrophil-activating peptide ENA-78 antibody
neutrophil-activating protein 78 antibody
Small inducible cytokine B5 antibody
small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) antibody
small inducible cytokine subfamily B, member 5 antibody
Small-inducible cytokine B5 antibody
Western blot - Anti-CXCL5 antibody (ab9983)
All lanes : Anti-CXCL5 antibody (ab9983)
Lane 1 : Marker Lane 2 : 250 ng Lane 3 : 125 ng Lane 4 : 62.5 ng Lane 5 : 31.25 ng Lane 6 : 15.625 ng Lane 7 : 7.8 ng Lane 8 : 3.9 ng Lane 9 : 1.95 ng Lane 10 : 0.975 ng Lane 11 : 0.4875 ng Lane 12 : 0.24 ng