Overview

  • Product nameCXCL7 Human ELISA Kit
    See all CXCL7 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 8.5 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.28 84% - 103%
    Serum 92.76 82% - 102%
    Plasma 93.45 83% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CXCL7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL7 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CXCL7 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceCXCL7 is a platelet-derived growth factor that belongs to the CXC chemokine family. It is a potent chemoattractant and activator of neutrophils and has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
  • Alternative names
    • B TG1
    • Beta TG
    • Beta thromboglobulin
    • C X C motif chemokine 7
    • Connective tissue activating peptide III
    • CTAP III
    • CTAP3
    • CTAPIII
    • LA PF4
    • LDGF
    • Leukocyte derived growth factor
    • Low affinity platelet factor IV
    • Macrophage derived growth factor
    • MDGF
    • NAP 2
    • Neutrophil activating peptide 2
    • PBP
    • Platelet basic protein
    • Ppbp
    • pro platelet basic protein (chemokine (C X C motif) ligand 7)
    • SCYB7
    • Small inducible cytokine B7
    • Small inducible cytokine subfamily B, member 7
    • TC1
    • TC2
    • TCK 1
    • TGB
    • TGB1
    • THBGB
    • THBGB1
    • Thrombocidin 1
    • Thrombocidin 2
    • Thymus Chemokine 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

CXCL7 Human ELISA Kit images

  • CXCL7 measured in biological fluids showing quantity (ng) per mL of tested sample

  • Representative Standard Curve using ab100613.

  • Representative Standard Curve using ab100613.

Protocols

References for CXCL7 Human ELISA Kit (ab100613)

ab100613 has not yet been referenced specifically in any publications.

Product Wall

The Assay Diluent B (1X) should be used to dilute the antibodies, regardless of what kind of samples are being assayed, plasma or other. Remember to dilute the diluent 5-fold (step 3): one part diluent stock and 4 parts deionized or distilled water.

Thank you for contacting us. The plates are from Greiner. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"