Overview

  • Product nameAnti-CXCR1 antibody [42705.111]
    See all CXCR1 primary antibodies
  • Description
    Mouse monoclonal [42705.111] to CXCR1
  • SpecificityHuman CXCR1 reacts with CXCR1 transfected cells and not with the parent cell line by flow cytometry. The antibody shows no cross-reactivity with human CXCR2.
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, Inhibition Assaymore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Human CXCR1 transfected NSO mouse myeloma cells.

  • General notes


    Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The alpha and beta families, with four cysteine residues, are the largest and best characterized. In the alpha family, one amino acid separates the first two cysteine residues (CXC); in the beta family the two cysteine residues (CC) are adjacent to each other. The alpha chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL8), while those that do not, act on lymphocytes (such as IP10 and MIG). Examples of chemokines under the beta family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the gamma family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the delta family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX3CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells. Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX3CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient. CXCR1 (IL8RA, or type I IL8 receptor) and CXCR2 (IL8RB, or type II IL8 receptor) have been shown to share approximately 77% amino acid sequence identity. IL8 binds to both receptors with high affinity and induces rapid elevation of cytosolic Ca 2+ levels. Whereas CXCR1 is highly specific for IL8, CXCR2 has broad specificity and has been shown to bind with high-affinity to other ELR motif containing a chemokines including GROs alpha, beta and gamma and NAP-2, and ENA-78. In contrast, PF4 and IP10 (two chemokines that lack the ELR motif) have been shown to lack binding affinity for CXCR2. CXCR1 and CXCR2 are expressed by neutrophils but not B lymphocytes or T lymphocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS
    Endotoxin level is < 10 ng per mg antibody as determined by the LAL method.
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThe antibody is purified from ascites fluid using protein G chromatography.
  • Primary antibody notesChemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The alpha and beta families, with four cysteine residues, are the largest and best characterized. In the alpha family, one amino acid separates the first two cysteine residues (CXC); in the beta family the two cysteine residues (CC) are adjacent to each other. The alpha chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL8), while those that do not, act on lymphocytes (such as IP10 and MIG). Examples of chemokines under the beta family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the gamma family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the delta family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX3CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells. Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX3CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient. CXCR1 (IL8RA, or type I IL8 receptor) and CXCR2 (IL8RB, or type II IL8 receptor) have been shown to share approximately 77% amino acid sequence identity. IL8 binds to both receptors with high affinity and induces rapid elevation of cytosolic Ca 2+ levels. Whereas CXCR1 is highly specific for IL8, CXCR2 has broad specificity and has been shown to bind with high-affinity to other ELR motif containing a chemokines including GROs alpha, beta and gamma and NAP-2, and ENA-78. In contrast, PF4 and IP10 (two chemokines that lack the ELR motif) have been shown to lack binding affinity for CXCR2. CXCR1 and CXCR2 are expressed by neutrophils but not B lymphocytes or T lymphocytes.
  • ClonalityMonoclonal
  • Clone number42705.111
  • MyelomaNS0
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 25 µg/ml. Tested on spleen with either fluorescent probes or chromogenic staining reagents.
Flow Cyt Use 10µl for 105-6 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use a concentration of 0.1 - 0.3 µg/ml.

Target

  • FunctionReceptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C C antibody
    • C C CKR 1 antibody
    • C-X-C chemokine receptor type 1 antibody
    • CCCKR1 antibody
    • CD 181 antibody
    • CD128 antibody
    • CD181 antibody
    • CD181 antigen antibody
    • CDw128a antibody
    • Chemokine (C X C motif) receptor 1 antibody
    • Chemokine (C X C) receptor 1 antibody
    • Chemokine receptor 1 antibody
    • CKR 1 antibody
    • CMKAR1 antibody
    • CXC-R1 antibody
    • CXCR 1 antibody
    • CXCR-1 antibody
    • CXCR1 antibody
    • CXCR1_HUMAN antibody
    • High affinity interleukin 8 receptor A antibody
    • High affinity interleukin-8 receptor A antibody
    • IL 8 receptor type 1 antibody
    • IL 8R A antibody
    • IL-8 receptor type 1 antibody
    • IL-8R A antibody
    • IL8 receptor antibody
    • IL8 receptor type 1 antibody
    • IL8R1 antibody
    • IL8RA antibody
    • IL8RBA antibody
    • Interleukin 8 receptor alpha antibody
    • Interleukin 8 receptor type 1 antibody
    • Interleukin 8 receptor type A antibody
    see all

References for Anti-CXCR1 antibody [42705.111] (ab10400)

This product has been referenced in:
  • Müller-Edenborn B  et al. Volatile anaesthetics reduce neutrophil inflammatory response by interfering with CXC receptor-2 signalling. Br J Anaesth N/A:N/A (2014). Human . Read more (PubMed: 24989774) »
  • Sánchez J  et al. The role of CXCR2 in systemic neovascularization of the mouse lung. J Appl Physiol 103:594-9 (2007). Flow Cyt ; Mouse . Read more (PubMed: 17556491) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.
We currently only have available the one size listed on the datasheet for a particular antibody. We do not have any CXCR antibody panels at this time.

I hope this information is helpful to you. Please do not...

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Je viens d'avoir la confirmation que les épitopes des deux anti-CXCR1 n'ont malheureusement pas été déterminés.

N'hésitez pas à me contacter de nouveau si vous avez d'autres questions.

Merci de nous avoir contactés.

Veuillez trouver ci-joints les 2 alignements de séquences, un pour le CXCR1, l'autre pour IL8.
Je suis en contact avec les laboratoires afin de savoir si les épitpes de ab10400 et ab89251...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (endothelial cells)
Specification endothelial cells
Permeabilization No
Username

Ms. Aigul Moldobaeva

Verified customer

Submitted Dec 06 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"