The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Use a concentration of 5 µg/ml.
Application notesIs unsuitable for IHC-P.
FunctionReceptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Post-translational modificationsPhosphorylated upon ligand binding; which is required for desensitization.
ICC/IF image of ab21641 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21641, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 5µg/ml.
Western blot - Anti-CXCR2 antibody (ab21641)
All lanes : Anti-CXCR2 antibody (ab21641) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Predicted band size : 41 kDa Observed band size : 41 kDa Additional bands at : 15 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
References for Anti-CXCR2 antibody (ab21641)
This product has been referenced in:
Jung JH et al. CXCR2 Inhibition in Human Pluripotent Stem Cells Induces Predominant Differentiation to Mesoderm and Endoderm Through Repression of mTOR, ß-Catenin, and hTERT Activities. Stem Cells Dev25:1006-19 (2016).
Read more (PubMed: 27188501) »