Overview

  • Product name
    Anti-CXCR2 (phospho S347) antibody
    See all CXCR2 primary antibodies
  • Description
    Rabbit polyclonal to CXCR2 (phospho S347)
  • Specificity
    Detects human CXCR2 only when phosphorylated at serine 347 (mouse: serine 346)
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized phosphopeptide derived from human CXCR2 around the phosphorylation site of serine 347 (R-P-SP-F-V).

  • Positive control
    • Breast carcinoma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21601983
WB Use at an assay dependent concentration. PubMed: 21601983
IHC-P 1/50 - 1/100.
ELISA 1/5000.

Target

  • Function
    Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    Phosphorylated upon ligand binding; which is required for desensitization.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C chemokine receptor type 2 antibody
    • CD 182 antibody
    • CD182 antibody
    • CD182 antigen antibody
    • CDw128b antibody
    • Chemokine (CXC) receptor 2 antibody
    • CMKAR2 antibody
    • CXC-R2 antibody
    • CXCR 2 antibody
    • CXCR-2 antibody
    • CXCR2 antibody
    • CXCR2_HUMAN antibody
    • GRO/MGSA receptor antibody
    • High affinity interleukin-8 receptor B antibody
    • IL 8 receptor type 2 antibody
    • IL 8R B antibody
    • IL-8 receptor type 2 antibody
    • IL-8R B antibody
    • IL8 RB antibody
    • IL8 receptor type 2 antibody
    • IL8R B antibody
    • IL8R2 antibody
    • IL8RA antibody
    • Interleukin 8 Receptor B antibody
    • Interleukin 8 receptor, beta antibody
    • Interleukin 8 receptor, type 2 antibody
    see all

Images

  • 1/50-1/100 ab61100 staining phosphorilated CXCR2 on human breast carcinoma tissue, in the presence or absence of the immunizing phospopeptide.

References

This product has been referenced in:
  • Halpern JL  et al. Mesenchymal stem cells promote mammary cancer cell migration in vitro via the CXCR2 receptor. Cancer Lett 308:91-9 (2011). WB, ICC/IF ; Mouse . Read more (PubMed: 21601983) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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