Anti-CXCR3 antibody [1C6] (ab125255)

Overview

  • Product nameAnti-CXCR3 antibody [1C6]
    See all CXCR3 primary antibodies
  • Description
    Mouse monoclonal [1C6] to CXCR3
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A 37 amino acid synthetic peptide corresponding to the N-terminus of Human CXCR3, conjugated to KLH.

  • Positive control
    • Human inflammable lymphocytes. CXCR3-transfected cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab125255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 40 kDa.
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.1 - 1 µg/ml.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionReceptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
    Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
    Isoform 3 mediates the activity of CXCL11.
  • Tissue specificityIsoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C chemokine receptor type 3 antibody
    • CD 183 antibody
    • CD182 antibody
    • CD183 antibody
    • Chemokine (C X C motif) receptor 3 antibody
    • Chemokine (C X C) receptor 3 antibody
    • Chemokine CXC Motif Receptor 3 antibody
    • CKR L2 antibody
    • CKR-L2 antibody
    • CKRL2 antibody
    • CMKAR3 antibody
    • CXC-R3 antibody
    • CXCR-3 antibody
    • CXCR3 antibody
    • CXCR3_HUMAN antibody
    • G Protein Coupled Receptor 9 antibody
    • G protein-coupled receptor 9 antibody
    • GPR9 antibody
    • Interferon-inducible protein 10 receptor antibody
    • IP-10 receptor antibody
    • IP10 antibody
    • IP10 R antibody
    • IP10 receptor antibody
    • IP10-R antibody
    • IP10R antibody
    • Mig R antibody
    • Mig receptor antibody
    • Mig-R antibody
    • MIGR antibody
    see all

Anti-CXCR3 antibody [1C6] images

  • ab125255, at 1/500 dilution, staining CXCR3 in formalin-fixed, paraffin-embedded Human inflammable lymphocytes.

References for Anti-CXCR3 antibody [1C6] (ab125255)

ab125255 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 09 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6
Permeabilization No
Specification Skin
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 09 2016

Thank you for contacting Abcam.

Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products

I think either of the two antibodies that you found would work well for you, as both are guaran...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"