Overview

  • Product nameAnti-CXCR3 antibody [2Ar1]
    See all CXCR3 primary antibodies
  • Description
    Mouse monoclonal [2Ar1] to CXCR3
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, Neutralising, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    NS0 cells transfected with human CXCR3.

Properties

Applications

Our Abpromise guarantee covers the use of ab64714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Neutralising Use a concentration of 1 - 6 µg/ml. in the presence of 7ng/ml of rhl-TAC, using the hCXCR3 transfected BaF/3 cells in a chemotaxis assay.
Flow Cyt Use a concentration of 5 - 10 µg/ml. Add 10µl to 1-2.5x105 cells in total reaction volume 200µl.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionReceptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
    Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
    Isoform 3 mediates the activity of CXCL11.
  • Tissue specificityIsoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C chemokine receptor type 3 antibody
    • CD 183 antibody
    • CD182 antibody
    • CD183 antibody
    • Chemokine (C X C motif) receptor 3 antibody
    • Chemokine (C X C) receptor 3 antibody
    • Chemokine CXC Motif Receptor 3 antibody
    • CKR L2 antibody
    • CKR-L2 antibody
    • CKRL2 antibody
    • CMKAR3 antibody
    • CXC-R3 antibody
    • CXCR-3 antibody
    • CXCR3 antibody
    • CXCR3_HUMAN antibody
    • G Protein Coupled Receptor 9 antibody
    • G protein-coupled receptor 9 antibody
    • GPR9 antibody
    • Interferon-inducible protein 10 receptor antibody
    • IP-10 receptor antibody
    • IP10 antibody
    • IP10 R antibody
    • IP10 receptor antibody
    • IP10-R antibody
    • IP10R antibody
    • Mig R antibody
    • Mig receptor antibody
    • Mig-R antibody
    • MIGR antibody
    see all

Anti-CXCR3 antibody [2Ar1] images

  • ab64714 staining CXCR3 in primary cultured HUVEC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/400 in PBS + 2.5% serum) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab64714 staining CXCR3 in pig uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to enzymatic antigen retrieval prior to blocking with 10% serum for 20 minutes at 24°C. The primary antibody was diluted 1/200 and incubated with the sample for 30 minutes at 24°C. A biotin-conjugated ABC kit was used to detect the antibody.

    See Abreview

  • ab64714 staining CXCR3 in Human glioblastoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer prior to blocking with a proprietary non-protein reagent for 5 minutes at 25°C. The primary antibody was diluted 1/2000 and incubated with the sample for 30 minutes. A biotin-conjugated goat anti-mouse IgG was used as the secondary antibody.

    See Abreview

References for Anti-CXCR3 antibody [2Ar1] (ab64714)

This product has been referenced in:
  • Tanaka A  et al. Th2 and regulatory immune reactions contribute to IgG4 production and the initiation of Mikulicz disease. Arthritis Rheum 64:254-63 (2012). IHC-P ; Human . Read more (PubMed: 21898360) »
  • Song CH  et al. Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prions. J Virol 85:11069-78 (2011). Read more (PubMed: 21813601) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: ER1
Permeabilization No
Specification Tonsil
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 03 2016

Application Flow Cytometry
Sample Cow Cell (lymphocytes)
Permeabilization No
Gating Strategy lymphocytes
Specification lymphocytes
Preparation Cell harvesting/tissue preparation method: Ficoll gradient
Sample buffer: Optimized PBS (BD Cellwash)
Fixation none
Username

Abcam user community

Verified customer

Submitted Jul 21 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (spleen, lymphe nodes)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0 / Tris-EDTA buffer pH 9.0 / Proteinase K
Permeabilization No
Specification spleen, lymphe nodes
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 21 2015

Thank you for contacting us.

Both antibodies use PBST or TBST at pH 7.4-7.6 in 5% milk.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your inquiry.

The immunogen for this antibody was the full length human CXCR3 (aa1-368, according to Accession # P49682).

To our knowledge the exact epitope was not further mapped.

I am sorry that I could not...

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Gracias por tu respuesta.
Si os interesa testar el anticuerpo en ratón, lo primero que tenéis que hacer es indicármelo para que yo os envíe el código canjeable por un anticuerpo gratuito en la siguiente compra.
Una vez te mande el código, pod...

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Thank you for contacting us.
Unfortunately we have not tested this antibody in mouse samples so do not have any experimental evidence of the use of it with this species.
The epitope is not mapped, so it is not possible to predict the extent o...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Glioblastoma)
Specification Glioblastoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step proprietary non-protein reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

Submitted Nov 30 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Uterus)
Specification Uterus
Fixative Paraformaldehyde
Antigen retrieval step Enzymatic
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Frank White

Verified customer

Submitted Oct 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Primary cultured HUVECs)
Specification Primary cultured HUVECs
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Bing Wang

Verified customer

Submitted Aug 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"