Overview

  • Product nameAnti-CXCR3 antibody
    See all CXCR3 primary antibodies
  • Description
    Rabbit polyclonal to CXCR3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human CXCR3.

    (Peptide available as ab74828.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Colon, Small Intestine, Lymph Node and Heart IF/ICC: MCF7 cell line

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionReceptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
    Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
    Isoform 3 mediates the activity of CXCL11.
  • Tissue specificityIsoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C chemokine receptor type 3 antibody
    • CD 183 antibody
    • CD182 antibody
    • CD183 antibody
    • Chemokine (C X C motif) receptor 3 antibody
    • Chemokine (C X C) receptor 3 antibody
    • Chemokine CXC Motif Receptor 3 antibody
    • CKR L2 antibody
    • CKR-L2 antibody
    • CKRL2 antibody
    • CMKAR3 antibody
    • CXC-R3 antibody
    • CXCR-3 antibody
    • CXCR3 antibody
    • CXCR3_HUMAN antibody
    • G Protein Coupled Receptor 9 antibody
    • G protein-coupled receptor 9 antibody
    • GPR9 antibody
    • Interferon-inducible protein 10 receptor antibody
    • IP-10 receptor antibody
    • IP10 antibody
    • IP10 R antibody
    • IP10 receptor antibody
    • IP10-R antibody
    • IP10R antibody
    • Mig R antibody
    • Mig receptor antibody
    • Mig-R antibody
    • MIGR antibody
    see all

Anti-CXCR3 antibody images

  • ICC/IF image of ab71864 stained formaldehyde cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CXCR3 antibody (ab71864) at 1 µg/ml

    Lane 1 : Human colon tissue lysate - total protein (ab30051)
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 4 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

    Our observed 70kDa band is supported by the following publications: http://www.jbc.org/content/279/29/30219.long and http://www.jimmunol.org/cgi/content/full/173/10/6234

References for Anti-CXCR3 antibody (ab71864)

ab71864 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Flow Cytometry
Sample Cow Cell (lymphocytes)
Permeabilization No
Gating Strategy lymphocytes
Specification lymphocytes
Preparation Cell harvesting/tissue preparation method: Ficoll gradient
Sample buffer: optimized PBS (BD Cellwash)
Fixation none
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Submitted Sep 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (spleen, lymphe nodes)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citratbuffer pH6.0 / Tris-EDTA pH 9.0
Permeabilization No
Specification spleen, lymphe nodes
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
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Submitted Aug 14 2015

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Mouse Cell lysate - whole cell (Endothelcells)
Specification Endothelcells
Treatment Ethanol und Dexametason 100nM 24h
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted May 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"