• Product nameAnti-CXCR4 antibody [44716.111]
    See all CXCR4 primary antibodies
  • Description
    Mouse monoclonal [44716.111] to CXCR4
  • SpecificityThis product reacts with human CXCR4 (fusin) transfected into a mouse cell line, and does not detect the endogenous mouse protein in the untransfected cells by flow cytometry. The antibody shows no cross-reactivity with other chemokine receptors. The antibody will neutralize human cell surface CXCR4 mediated SDF/ PBSF response using human cultured lymphocytes in a chemotaxis assay.
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-FoFr, Flow Cyt, Inhibition Assay, Neutralisingmore details
  • Species reactivity
    Reacts with: Cat, Human
    Does not react with: Rat
  • Immunogen

    Human CXCR4 transfected NSO mouse myeloma cells.

  • Positive control
    • ICC: stimulated human peripheral blood mononuclear cells (PBMC) Inhib: BaF/3 human CXCR-4 transfected cells



Our Abpromise guarantee covers the use of ab10403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.5 - 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml. Fix with 2% formaldehyde and permeabilize with 0.1% saponin.
IHC-FoFr Use at an assay dependent concentration.
Flow Cyt Use 10µg for 106 cells. (see Endres et al reference).

ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use a concentration of 2.5 - 20 µg/ml. (this is the ND50 where the antibody will block 50% of the bioactivity due to 0.1 ug/ml recombinant human SDF-1 in an assay measuring chemotaxis using BaF/3 human CXCR-4 transfected cells).
Neutralising Use at an assay dependent concentration. PubMed: 22995914


  • FunctionReceptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ions levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhance intracellular calcium ions and reduce cellular cAMP levels. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.
  • Tissue specificityExpressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
  • Involvement in diseaseDefects in CXCR4 are a cause of WHIM syndrome (WHIM) [MIM:193670]; also known as warts, hypogammaglobulinemia, infections and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • DomainThe amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity.
  • Post-translational
    Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation.
    Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S.
    Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization.
    O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity.
  • Cellular localizationCell membrane. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C chemokine receptor type 4 antibody
    • CD184 antibody
    • CD184 antigen antibody
    • Chemokine (C X C motif) receptor 4 antibody
    • Chemokine CXC Motif Receptor 4 antibody
    • CXC-R4 antibody
    • CXCR-4 antibody
    • CXCR4 antibody
    • CXCR4_HUMAN antibody
    • D2S201E antibody
    • FB22 antibody
    • Fusin antibody
    • HM89 antibody
    • HSY3RR antibody
    • LAP 3 antibody
    • LAP3 antibody
    • LCR1 antibody
    • LESTR antibody
    • Leukocyte derived seven transmembrane domain receptor antibody
    • Leukocyte-derived seven transmembrane domain receptor antibody
    • Lipopolysaccharide associated protein 3 antibody
    • Neuropeptide Y receptor Y3 antibody
    • NPY3R antibody
    • NPYR antibody
    • NPYRL antibody
    • NPYY3 antibody
    • NPYY3R antibody
    • Probable G protein coupled receptor lcr1 homolog antibody
    • SDF 1 receptor antibody
    • SDF-1 receptor antibody
    • Stromal cell derived factor 1 receptor antibody
    • Stromal cell-derived factor 1 receptor antibody
    • WHIM antibody
    • WHIMS antibody
    see all

References for Anti-CXCR4 antibody [44716.111] (ab10403)

This product has been referenced in:
  • Nobutani K  et al. Downregulation of CXCR4 in Metastasized Breast Cancer Cells and Implication in Their Dormancy. PLoS One 10:e0130032 (2015). Read more (PubMed: 26083776) »
  • Singh AP  et al. CXCL12/CXCR4 protein signaling axis induces sonic hedgehog expression in pancreatic cancer cells via extracellular regulated kinase- and Akt kinase-mediated activation of nuclear factor ?B: implications for bidirectional tumor-stromal interactions. J Biol Chem 287:39115-24 (2012). Neutralising ; Human . Read more (PubMed: 22995914) »

See all 5 Publications for this product

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The number of tests totally depends on the dilution or concentration range customer uses. However at the recommended dilution, the antibody can be used for 100 ICC/IF or 100 IHC-P or 100 WB tests.

I hope t...

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It looks quite good for the cross-reactivity withrabbit inboth antibodies:

ab10403 Anti-CXCR4 antibody [44716.111]: the protein and the immunogen have a similarity of 97%

ab86371 Anti-GATA4 ant...

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Unfortunately, the immunogen of ab1671 shows less than 60% homology with dog and therefore we do not expect a crpss-raction of this antibody. ab63851 does show a 93% homology with dog and would in general be eligab...

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Thank you for your enquery. Yes, the antibody should detect the endogenous human protein (which is human Fusin). The parent cell line was a mouse cell line transfected with human fusin. Thus, to clarify the comment on FACS analysis: It will detect ...

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I'm afraid the epitope for the antibody ab10403 has not been mapped, we therefore do not know where the antibody binds on the receptor. Could I please suggest sharing your positive experience in FACS with ab10403 with other users by submitting an A...

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