Anti-CXCR4 (phospho S339) antibody (ab74012)

Overview

  • Product nameAnti-CXCR4 (phospho S339) antibody
    See all CXCR4 primary antibodies
  • Description
    Rabbit polyclonal to CXCR4 (phospho S339)
  • Specificityab74012 detects endogenous levels of CXCR4 only when phosphorylated at serine 339 (Mouse: Ser 346).
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human CXCR4 around the phosphorylation site of serine 339 (H-S-SP-V-S).

  • Positive control
    • Human brain carcinoma tissue. Extracts from HUVEC cells treated with etoposide (25uM, 24hours). HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionReceptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ions levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhance intracellular calcium ions and reduce cellular cAMP levels. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.
  • Tissue specificityExpressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
  • Involvement in diseaseDefects in CXCR4 are a cause of WHIM syndrome (WHIM) [MIM:193670]; also known as warts, hypogammaglobulinemia, infections and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • DomainThe amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity.
  • Post-translational
    modifications
    Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation.
    Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S.
    Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization.
    O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity.
  • Cellular localizationCell membrane. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C chemokine receptor type 4 antibody
    • CD184 antibody
    • CD184 antigen antibody
    • Chemokine (C X C motif) receptor 4 antibody
    • Chemokine CXC Motif Receptor 4 antibody
    • CXC-R4 antibody
    • CXCR-4 antibody
    • CXCR4 antibody
    • CXCR4_HUMAN antibody
    • D2S201E antibody
    • FB22 antibody
    • Fusin antibody
    • HM89 antibody
    • HSY3RR antibody
    • LAP 3 antibody
    • LAP3 antibody
    • LCR1 antibody
    • LESTR antibody
    • Leukocyte derived seven transmembrane domain receptor antibody
    • Leukocyte-derived seven transmembrane domain receptor antibody
    • Lipopolysaccharide associated protein 3 antibody
    • Neuropeptide Y receptor Y3 antibody
    • NPY3R antibody
    • NPYR antibody
    • NPYRL antibody
    • NPYY3 antibody
    • NPYY3R antibody
    • Probable G protein coupled receptor lcr1 homolog antibody
    • SDF 1 receptor antibody
    • SDF-1 receptor antibody
    • SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR antibody
    • Stromal cell derived factor 1 receptor antibody
    • Stromal cell-derived factor 1 receptor antibody
    • WHIM antibody
    • WHIMS antibody
    see all

Anti-CXCR4 (phospho S339) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human brain carcinoma tissue using ab74012, at 1/50 dilution, in the presence (right panel) or absence (left panel) of the immunising phosphopeptide.
  • All lanes : Anti-CXCR4 (phospho S339) antibody (ab74012) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells treated with etoposide (25uM, 24hours)
    Lane 2 : Extracts from HUVEC cells treated with etoposide (25uM, 24hours) with the immunising phosphopeptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of HeLa cells using ab74012, at 1/500 dilution, in the presence (right panel) or absence (left panel) of the immunising phosphopeptide.

References for Anti-CXCR4 (phospho S339) antibody (ab74012)

This product has been referenced in:
  • Pitcher J  et al. Neuronal ferritin heavy chain and drug abuse affect HIV-associated cognitive dysfunction. J Clin Invest 124:656-69 (2014). WB, IHC-P ; Human, Rhesus monkey . Read more (PubMed: 24401274) »
  • Buss MC  et al. The WIP1 oncogene promotes progression and invasion of aggressive medulloblastoma variants. Oncogene 0:N/A (2014). Read more (PubMed: 24632620) »

See all 8 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Jul 30 2014

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab74012 in chicken. This code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please s...

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Thank you for your inquiry.

I can confirm that both antibodies are tested and guaranteed for mouse and IHC-P.

They do bind to different epitopes in the CXCR4 protein and ab74012 will only bind to the CXCR4 protein when it is phospho...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with the anti-CXCR4 (phospho S339) antibody (ab74012). We take product complaints very seriously, and investigate every product that we feel may not be perform...

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Thank you for your response and for providing some further details.

I would like to clarify a few points in this e-mail.

This (ab74012: Anti-CXCR4 (phospho S339)) is a polyclonal antibody (not a monoclonal) and will therefore consis...

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Thank you for getting back to me and for providing some further details.

This antibody detects endogenous levels of CXCR4 only when phosphorylated at serine 339 (Mouse: Ser 346).

After reading through the detailed protocol you kindl...

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Thank you for your enquiry regarding ab74012 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

The immunogen used to raise this antibody was a s...

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Application Western blot
Sample Rat Cell lysate - whole cell (Mesenchymal stem cell)
Loading amount 15 µg
Specification Mesenchymal stem cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"