Overview

  • Product nameAnti-CXCR5 antibody
    See all CXCR5 primary antibodies
  • Description
    Rabbit polyclonal to CXCR5
  • Tested applicationsSuitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide corresponding to a region near the N terminus of Human CXCR5

Properties

Applications

Our Abpromise guarantee covers the use of ab19017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 41 kDa).

Target

  • FunctionCytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
  • Tissue specificityExpression in mature B-cells and Burkitt lymphoma cells.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BLR 1 antibody
    • BLR1 antibody
    • Burkitt lymphoma receptor 1 antibody
    • Burkitt lymphoma receptor 1 GTP binding protein antibody
    • Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5) antibody
    • C X C chemokine receptor type 5 antibody
    • C-X-C chemokine receptor type 5 antibody
    • CD 185 antibody
    • CD185 antibody
    • CD185 antigen antibody
    • Chemokine (C-X-C motif) receptor 5 antibody
    • Chemokine C X C motif receptor 5 antibody
    • Chemokine CXC motif receptor 5 antibody
    • Chemokine receptor 5 antibody
    • CXC chemokine receptor type 5 antibody
    • CXC R5 antibody
    • CXC-R5 antibody
    • CXCR 5 antibody
    • CXCR-5 antibody
    • Cxcr5 antibody
    • CXCR5_HUMAN antibody
    • MDR 15 antibody
    • MDR-15 antibody
    • MDR15 antibody
    • MGC117347 antibody
    • Monocyte derived receptor 15 antibody
    • Monocyte-derived receptor 15 antibody
    see all

Anti-CXCR5 antibody images

  • Flow cytometry analysis of CXCR5 transfected HEK293 cells, staining CXCR5 with ab19017.

    The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA + 0.01% Azide) for 1 hour at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (200) was used as the secondary antibody.

    Gating Strategy: Live cells

    See Abreview

References for Anti-CXCR5 antibody (ab19017)

ab19017 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Dog Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive Lymphocytes
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Submitted Nov 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (human CXCR5 transfected HEK)
Specification human CXCR5 transfected HEK
Permeabilization No
Gating Strategy alive cells
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Verified customer

Submitted Nov 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
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Submitted Apr 19 2012

Application Flow Cytometry
Sample Rat Cell (spleen)
Specification spleen
Preparation Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive lymphocytes
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Submitted Apr 19 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spleen from immune mouse)
Specification Spleen from immune mouse
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Apr 18 2012

Application Flow Cytometry
Sample Mouse Cell (splenocytes)
Specification splenocytes
Preparation Cell harvesting/tissue preparation method: after spleen mashing
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive B cells
Username

Abcam user community

Verified customer

Submitted Apr 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"