The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 41 kDa).
FunctionCytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
Tissue specificityExpression in mature B-cells and Burkitt lymphoma cells.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Flow Cytometry - Anti-CXCR5 antibody (ab19017)This image is courtesy of an anonymous Abreview
Flow cytometry analysis of CXCR5 transfected HEK293 cells, staining CXCR5 with ab19017.
The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA + 0.01% Azide) for 1 hour at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (200) was used as the secondary antibody.