Overview

  • Product nameAnti-CXCR5 antibody
    See all CXCR5 primary antibodies
  • Description
    Rabbit polyclonal to CXCR5
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CXCR5.

    (Peptide available as ab46217.)

  • Positive control
    • ab46218 gave a positive signal in the following whole cell lysates: Ramos; MOLT4; Raji; Daudi. ab46218 also gave a positive signal in Human Lymph Node tissue lysate (data not shown)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab46218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human CXCR5 peptide (ab46217).
IHC-P Use a concentration of 1 µg/ml.

Target

  • FunctionCytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
  • Tissue specificityExpression in mature B-cells and Burkitt lymphoma cells.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BLR 1 antibody
    • BLR1 antibody
    • Burkitt lymphoma receptor 1 antibody
    • Burkitt lymphoma receptor 1 GTP binding protein antibody
    • Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5) antibody
    • C X C chemokine receptor type 5 antibody
    • C-X-C chemokine receptor type 5 antibody
    • CD 185 antibody
    • CD185 antibody
    • CD185 antigen antibody
    • Chemokine (C-X-C motif) receptor 5 antibody
    • Chemokine C X C motif receptor 5 antibody
    • Chemokine CXC motif receptor 5 antibody
    • Chemokine receptor 5 antibody
    • CXC chemokine receptor type 5 antibody
    • CXC R5 antibody
    • CXC-R5 antibody
    • CXCR 5 antibody
    • CXCR-5 antibody
    • Cxcr5 antibody
    • CXCR5_HUMAN antibody
    • MDR 15 antibody
    • MDR-15 antibody
    • MDR15 antibody
    • MGC117347 antibody
    • Monocyte derived receptor 15 antibody
    • Monocyte-derived receptor 15 antibody
    see all

Anti-CXCR5 antibody images

  • All lanes : Anti-CXCR5 antibody (ab46218) at 1 µg/ml

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : Daudi whole cell lysate (ab3951)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab46218 staining CXCR5 in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) second test for 20 mins. The section was then incubated with ab46218, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-CXCR5 antibody (ab46218)

ab46218 has not yet been referenced specifically in any publications.

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The lab let me know that our antigen retrieval solution comes pre-made up from another company and they state it as an ‘EDTA based pH9 solution’. They do not give out much information in regards towha...

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