Anti-Cy3 / Cy5 antibody [CY96] (ab52060)

Overview

• Product nameAnti-Cy3 / Cy5 antibody [CY96]
• Description
  Mouse monoclonal [CY96] to Cy3 / Cy5
• Specificityab52060 recognizes Cy3, Cy5, AlexaFluor 647 and AlexaFluor 547 conjugated to proteins.
• Tested applicationsSuitable for: Dot blot, ELISA, IP, ICC, Other, ICC/IFmore details
• Immunogen

  A mixture of proteins labelled with Cy3 or Cy5.

• General notes

  
  

  Monoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: 0.01M PBS, pH 7.4
• Concentration information loading...
• Purification notesPurified Mouse Immunoglobulin
• Primary antibody notesMonoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.
• ClonalityMonoclonal
• Clone numberCY96
• IsotypeIgG2a
• Research areas
  • Tags & Cell Markers
  • Epitope Tags
  • Conjugates

Associated products

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

Target

• RelevanceCy3 and Cy5 are reactive watersoluble fluorescent dyes of the cyanine dye family used for labelling proteins, peptides, DNA, RNA, and other biomolecules. They are usually synthesized with reactive groups on either one or both of the nitrogen side chains so that they can be chemically cross-linked to nucleic acids or protein molecules. Thay are small, pH insensitive (between pH 3 and pH 10), soluble in aqueous solution and have tolerance to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. They are used in many different biological assays, including DNA microarrays, protein microarrays, two-dimensional protein analysis, fluorescence resonance energy transfer, and immunocytochemistry. Cy3 is excited maximally at 550 nm and emits maximally at 570 nm, in the green part of the spectrum. Cy5 is excited maximally at 649 nm and emits maximally at 670 nm, in the red part of the spectrum.