• Product name
  • Description
    Rabbit polyclonal to CYB5R3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 191-240 (IRAIMKDPDD HTVCHLLFAN QTEKDILLRP ELEELRNKHS ARFKLWYTLD) of Human CYB5R3 (NP_000389).

  • Positive control
    • HT1080 cell lysate.



Our Abpromise guarantee covers the use of ab113222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
  • Tissue specificity
    Isoform 2 is expressed at late stages of erythroid maturation.
  • Involvement in disease
    Defects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3) [MIM:250800]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well-tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms.
  • Sequence similarities
    Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 FAD-binding FR-type domain.
  • Cellular localization
    Endoplasmic reticulum membrane. Mitochondrion outer membrane and Cytoplasm. Produces the soluble form found in erythrocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • B5R antibody
    • Cyb5r3 antibody
    • Cytochrome b5 reductase 3 antibody
    • Cytochrome b5 reductase antibody
    • DIA1 antibody
    • Diaphorase 1 antibody
    • Diaphorase-1 antibody
    • NADH cytochrome b5 reductase 3 antibody
    • NADH-cytochrome b5 reductase 3 membrane-bound form antibody
    • NADH-cytochrome b5 reductase 3 soluble form antibody
    • NB5R3_HUMAN antibody
    • OTTHUMP00000028761 antibody
    • OTTHUMP00000198435 antibody
    • OTTHUMP00000198574 antibody
    • OTTHUMP00000198662 antibody
    • OTTHUMP00000198665 antibody
    see all

Anti-CYB5R3 antibody images

  • Anti-CYB5R3 antibody (ab113222) at 1 µg/ml + HT1080 cell lysate at 10 µg

    Predicted band size : 34 kDa

References for Anti-CYB5R3 antibody (ab113222)

ab113222 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab113222.
Please use the links above to contact us or submit feedback about this product.


Sign up