Overview

  • Product nameAnti-CYB5R3 antibody
    See all CYB5R3 primary antibodies
  • Description
    Mouse polyclonal to CYB5R3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length CYB5R3 protein (Human)

  • Positive control
    • WB: CYB5R3 transfected lysate ICC/IF: Hela cell

Properties

Applications

Our Abpromise guarantee covers the use of ab67526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionDesaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
  • Tissue specificityIsoform 2 is expressed at late stages of erythroid maturation.
  • Involvement in diseaseDefects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3) [MIM:250800]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well-tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms.
  • Sequence similaritiesBelongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 FAD-binding FR-type domain.
  • Cellular localizationEndoplasmic reticulum membrane. Mitochondrion outer membrane and Cytoplasm. Produces the soluble form found in erythrocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • B5R antibody
    • Cyb5r3 antibody
    • Cytochrome b5 reductase 3 antibody
    • Cytochrome b5 reductase antibody
    • DIA1 antibody
    • Diaphorase 1 antibody
    • Diaphorase-1 antibody
    • NADH cytochrome b5 reductase 3 antibody
    • NADH-cytochrome b5 reductase 3 membrane-bound form antibody
    • NADH-cytochrome b5 reductase 3 soluble form antibody
    • NB5R3_HUMAN antibody
    • OTTHUMP00000028761 antibody
    • OTTHUMP00000198435 antibody
    • OTTHUMP00000198574 antibody
    • OTTHUMP00000198662 antibody
    • OTTHUMP00000198665 antibody
    see all

Anti-CYB5R3 antibody images

  • All lanes : Anti-CYB5R3 antibody (ab67526) at 1/500 dilution

    Lane 1 : CYB5R3 transfected lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • ab67526, at 10µg/ml, staining CYB5R3 in Hela cell by Immunofluorescence.

References for Anti-CYB5R3 antibody (ab67526)

ab67526 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam regarding ab67526.

The immunogen used to generate this antibody is full length CYB5R3. Since it is a polyclonal antibody, it will recognize many regions along the length of this protein. We have not specificall...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"