Anti-CYB5R3 antibody [EPR3867] (ab109620)

Overview

  • Product nameAnti-CYB5R3 antibody [EPR3867]
    See all CYB5R3 primary antibodies
  • Description
    Rabbit monoclonal [EPR3867] to CYB5R3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human CYB5R3.

  • Positive control
    • A431, HepG2, and HeLa cell lysates. Human testis tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
IHC-P 1/250 - 1/500. (Heat to 98°C, allow to cool for 10-20 minutes)
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionDesaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
    • Tissue specificityIsoform 2 is expressed at late stages of erythroid maturation.
    • Involvement in diseaseDefects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3) [MIM:250800]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well-tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms.
    • Sequence similaritiesBelongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
      Contains 1 FAD-binding FR-type domain.
    • Cellular localizationEndoplasmic reticulum membrane. Mitochondrion outer membrane and Cytoplasm. Produces the soluble form found in erythrocytes.
    • Information by UniProt
    • Database links
    • Alternative names
      • B5R antibody
      • Cyb5r3 antibody
      • Cytochrome b5 reductase 3 antibody
      • Cytochrome b5 reductase antibody
      • DIA1 antibody
      • Diaphorase 1 antibody
      • Diaphorase-1 antibody
      • NADH cytochrome b5 reductase 3 antibody
      • NADH-cytochrome b5 reductase 3 membrane-bound form antibody
      • NADH-cytochrome b5 reductase 3 soluble form antibody
      • NB5R3_HUMAN antibody
      • OTTHUMP00000028761 antibody
      • OTTHUMP00000198435 antibody
      • OTTHUMP00000198574 antibody
      • OTTHUMP00000198662 antibody
      • OTTHUMP00000198665 antibody
      see all

    Anti-CYB5R3 antibody [EPR3867] images

    • All lanes : Anti-CYB5R3 antibody [EPR3867] (ab109620) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
    • ab109620 at 1/250 dilution staining CYB5R3 in paraffin-embedded Human testis tissue by Immunohistochemistry.

    References for Anti-CYB5R3 antibody [EPR3867] (ab109620)

    ab109620 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your inquiry.
    For ab109620, the exact immunogen shares 79% identity with CYB5R2. This antibody is not predicted to recognize CYB5R2, due to low sequence homology but we haven't specifically tested this.
    We do have an CYB5R3 prot...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"