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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A antibody (ab7956)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A antibody (ab7956)
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Product Name 

Cyclin A antibody

 

See all Cyclin A antibodies (18)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Cyclin A

Immunogen 

Synthetic peptide: HSKYHSVSLLNPPETLSV, corresponding to C terminal amino acids 405-422 of Mouse Cyclin A.
BLAST 'HSKYHSVSLLNPPETLSV' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Reacts with cyclin A2.

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, IP, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• IHC-P

1 Abreview

- average rating: 3 stars (out of 5)3 stars (out of 5)3 stars (out of 5)3 stars (out of 5)3 stars (out of 5)

• WB

1 Abreview

 


IHC-P
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution (PMID 20133761).
IHC-P: 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (see Abreview).
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 49 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

K562

Cellular localization 

Nuclear

Research areas 

Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins >> Apolipoproteins
Cancer >> Cell cycle >> Cyclins >> Cyclin A family
Chromatin and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin A Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin A Family

Relevance 

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2 phase.

 

Alternative names Cyclin A antibody (ab7956)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

890    (Human)
12428    (Mouse)
114494    (Rat)

Omim

    

123835    (Human)

SwissProt

    

P20248    (Human)
P51943    (Mouse)

Unigene

    

58974    (Human)
13094    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

PBS with 0.1% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cyclin A antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Cyclin A antibody images, references, products related to ab7956 and other tools.

 

Cyclin A antibody images:

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A antibody (ab7956)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A antibody (ab7956)

ab7956 at 1/150 staining developing mouse embryo tissue sections (5 microns) by IHC-P. The tissue was formaldehyde fixed and and heat mediated antigen retrieval step in citric acid buffer was performed. The tissue was blocked in BSA and incubated with the antibody for 2 hours. A biotinylated goat antibody was used as the secondary. The image shows T/S of developing oesophagus, trachea, skeletal muscle and blood vessel wall.

This image is courtesy of an Abreview submitted by Mr Carl hobbs

See Abreview

 

References for Cyclin A antibody (ab7956)

Specific publication references   This product has been used in: (two most recent references)

Pascreau G et al. Discovery of a distinct domain in cyclin A sufficient for centrosomal localization independently of Cdk binding. Proc Natl Acad Sci U S A 107:2932-7 (2010). ICC/IF; Human. PubMed: 20133761

Sebastian S et al. MLL5, a trithorax homolog, indirectly regulates H3K4 methylation, represses cyclin A2 expression, and promotes myogenic differentiation. Proc Natl Acad Sci U S A 106:4719-24 (2009). WB; Mouse. PubMed: 19264965


See all 12 publication references for this product.


If you publish research using ab7956 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Cyclin A

 

Cyclin A antibody - more information

Customer reviews (feedback) regarding Cyclin A antibody

Customer FAQs regarding Cyclin A antibody

Protocols for Cyclin A antibody

Price and availability of products related to Cyclin A antibody

 

Cyclin A antibody - related products:

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Loading controls

Lysates

 

Immunizing Peptide for Cyclin A antibody

ab8011

  

Cyclin A (C-term) peptide (mouse)

 

Positive Controls for Cyclin A antibody

ab7911

  

K-562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

 

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Other Cyclin A Antibodies

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Cyclin A antibody - other tools:

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