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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A antibody (ab7956)
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Product Name
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Cyclin A antibody
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See all Cyclin A antibodies (18)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cyclin A
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Immunogen
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Synthetic peptide: HSKYHSVSLLNPPETLSV, corresponding to C terminal amino acids 405-422 of Mouse Cyclin A.
BLAST 'HSKYHSVSLLNPPETLSV' with  or 
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Reacts with
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Hu, Ms, Rat
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Specificity
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Reacts with cyclin A2.
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution (PMID 20133761).
IHC-P: 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (see Abreview).
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 49 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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K562
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Cellular localization
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Nuclear
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Research areas
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Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins >> Apolipoproteins
Cancer >> Cell cycle >> Cyclins >> Cyclin A family
Chromatin and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin A Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin A Family
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Relevance
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Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2 phase.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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PBS with 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cyclin A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cyclin A antibody images, references, products related to ab7956 and other tools.
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Cyclin A antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A antibody (ab7956)
ab7956 at 1/150 staining developing mouse embryo tissue sections (5 microns) by IHC-P. The tissue was formaldehyde fixed and and heat mediated antigen retrieval step in citric acid buffer was performed. The tissue was blocked in BSA and incubated with the antibody for 2 hours. A biotinylated goat antibody was used as the secondary. The image shows T/S of developing oesophagus, trachea, skeletal muscle and blood vessel wall. This image is courtesy of an Abreview submitted by Mr Carl hobbs
See Abreview
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References for Cyclin A antibody (ab7956)
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This product has been used in: (two most recent references)
Pascreau G et al. Discovery of a distinct domain in cyclin A sufficient for centrosomal localization independently of Cdk binding. Proc Natl Acad Sci U S A 107:2932-7 (2010). ICC/IF; Human. PubMed: 20133761
Sebastian S et al. MLL5, a trithorax homolog, indirectly regulates H3K4 methylation, represses cyclin A2 expression, and promotes myogenic differentiation. Proc Natl Acad Sci U S A 106:4719-24 (2009). WB; Mouse. PubMed: 19264965
See all 12 publication references for this product.
If you publish research using ab7956 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Cyclin A
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Cyclin A antibody - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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