Anti-Cyclin A1 antibody (ab133183)
Key features and details
- Rabbit polyclonal to Cyclin A1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cyclin A1 antibody
See all Cyclin A1 primary antibodies -
Description
Rabbit polyclonal to Cyclin A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide from the C-terminal region of Human Cyclin A1.
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Positive control
- SKOV3 cell extract; Human testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 52 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 52 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. -
Tissue specificity
Very high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Expression increases in early G1 phase and reaches highest levels during the S and G2/M phases. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8900 Human
- Entrez Gene: 12427 Mouse
- Entrez Gene: 295052 Rat
- Omim: 604036 Human
- SwissProt: P78396 Human
- SwissProt: Q61456 Mouse
- SwissProt: Q6AY13 Rat
- Unigene: 417050 Human
see all -
Alternative names
- CCN A1 antibody
- CCNA 1 antibody
- Ccna1 antibody
see all
Images
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All lanes : Anti-Cyclin A1 antibody (ab133183) at 1/500 dilution
Lane 1 : SKOV3 cell extract
Lane 2 : SKOV3 cell extract with synthesized
peptide
Predicted band size: 52 kDa -
Immunohistochemical analysis of paraffin embedded Human testis tissue labelling Cyclin A1 with ab133183 antibody at a concentration of 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab133183 has been referenced in 4 publications.
- Li S & Tu H Psoralen inhibits the proliferation and promotes apoptosis through endoplasmic reticulum stress in human osteosarcoma cells. Folia Histochem Cytobiol 60:101-109 (2022). PubMed: 35257357
- Wu Y et al. The Transcriptional Cell Atlas of Testis Development in Sheep at Pre-Sexual Maturity. Curr Issues Mol Biol 44:483-497 (2022). PubMed: 35723319
- Wang F et al. Degradation of CCNB1 mediated by APC11 through UBA52 ubiquitination promotes cell cycle progression and proliferation of non-small cell lung cancer cells. Am J Transl Res 11:7166-7185 (2019). PubMed: 31814919
- Li F et al. CEP55 promotes cell proliferation and inhibits apoptosis via the PI3K/Akt/p21 signaling pathway in human glioma U251 cells. Oncol Lett 15:4789-4796 (2018). PubMed: 29552118