Overview

  • Product nameAnti-Cyclin A1 antibody
    See all Cyclin A1 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin A1
  • SpecificityDetects endogenous levels of total Cyclin A1 protein.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • SKOV3 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab53699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA 1/40000.
IHC-P Use a concentration of 1 µg/ml.

Target

  • FunctionMay be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
  • Tissue specificityVery high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stageExpression increases in early G1 phase and reaches highest levels during the S and G2/M phases.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCN A1 antibody
    • CCNA 1 antibody
    • Ccna1 antibody
    • CCNA1_HUMAN antibody
    • Cyclin-A1 antibody
    • CyclinA1 antibody
    • G2/mitotic specific cyclin A1 antibody
    • MGC132235 antibody
    • MGC159139 antibody
    see all

Anti-Cyclin A1 antibody images

  • All lanes : Anti-Cyclin A1 antibody (ab53699) at 1/500 dilution

    Lane 1 : SKOV3 cell extract
    Lane 2 : SKOV3 cell extract with immunising peptide


    Predicted band size : 52 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab53699 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53699, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab53699 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53699, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Cyclin A1 antibody (ab53699)

This product has been referenced in:
  • Pakula A  et al. Altered expression of cyclin A 1 in muscle of patients with facioscapulohumeral muscle dystrophy (FSHD-1). PLoS One 8:e73573 (2013). ICC/IF . Read more (PubMed: 24019929) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium citrate pH 6.0
Sample Mouse Tissue sections (Testes)
Specification Testes
Blocking step Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Manju Sharma

Verified customer

Submitted Mar 19 2015

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