Recombinant
RabMAb

Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)

Overview

  • Product name
    Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054]
    See all Cyclin A1 + Cyclin A2 primary antibodies
  • Description
    Rabbit monoclonal [EPR18054] to Cyclin A1 + Cyclin A2
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Cyclin A1 + Cyclin A2 aa 250-450. The exact sequence is proprietary. Immunogen is from Cyclin A1. Cyclin A2: P20248
    Database link: P78396

  • Positive control
    • WB: Recombinant fragment of Human Cyclin A1 protein; Full length Human Cyclin A2 recombinant protein; Human testis lysate. HeLa and HepG2 whole cell lysate. IHC-P: Human testis and colon cancer tissues. IP: K562 whole cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-Cyclin A1 + Cyclin A2 antibody (Alexa Fluor® 488) [EPR18054] (ab206746)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR18054
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab185619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 52, 49 kDa (predicted molecular weight: 52, 49 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/70.

Target

  • Relevance
    Cyclin A1: Function: May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Tissue specificity: Very high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines. Similarity: Belongs to the cyclin family. Cyclin AB subfamily. Developmental stage: Expression increases in early G1 phase and reaches highest levels during the S and G2/M phases. Cyclin A2: Function: Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. Similarity: Belongs to the cyclin family. Cyclin AB subfamily. Developmental stage: Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Cellular localization
    Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • CCN1 antibody
    • CCNA antibody
    • CCNA1 antibody
    • CCNA2 antibody
    • CT146 antibody
    • Cyclin-A antibody
    • Cyclin-A1 antibody
    • Cyclin-A2 antibody
    see all

Images

  • Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/20000 dilution + Recombinant fragment of Human Cyclin A1 protein at 0.01 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 52, 49 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 seconds

    Recombinant fragment of Human Cyclin A1 protein contains aa261-450 with His-Tag & GST-tag.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Cyclin A1 + Cyclin A2 using ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining of spermatocytes of the human testis is observed. Counterstained with Hematoxylin.
    Negative control obtained using PBS instead of ab185619 and secondary antibody only.

  • Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/2000 dilution + Full length Human Cyclin A2 recombinant protein at 0.01 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 52, 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    Recombinant full length Cyclin A2 protein contains aa1-432 with His-Tag.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer, labeling Cyclin A1 + Cyclin A2 with ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining of human colonic adenocarcinoma cells is observed. Counterstained with Hematoxylin.
    Negative control obtained using PBS instead of ab185619 and secondary antibody only.

  • Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 52, 49 kDa
    Observed band size : kDa


    Exposure time : 1 minute

    5% NFDM/TBST: Blocking and diluting buffer.

  • Cyclin A1 + Cyclin A2 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab185619 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab185619 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: K562 whole cell extract 10 µg (Input).

    Lane 2: ab185619 IP in K562 whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185619 in K562 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds

  • All lanes : Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 52, 49 kDa
    Observed band size : kDa


    Exposure time : 3 minutes

    5% NFDM/TBST: Blocking and diluting buffer.

References

This product has been referenced in:
  • Tseng YH  et al. Thyroid hormone suppresses expression of stathmin and associated tumor growth in hepatocellular carcinoma. Sci Rep 6:38756 (2016). Human . Read more (PubMed: 27934948) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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