Overview

  • Product nameAnti-Cyclin A2 antibody [6E6]
    See all Cyclin A2 primary antibodies
  • Description
    Mouse monoclonal [6E6] to Cyclin A2
  • SpecificityThis antibody may be used to examine the biological behavior of tumors. Cyclins are proteins that vary in abundance and are associated with and activate cyclin dependent kinases at different stages of the cell cycle. Cyclin A is detectable in S phase increasing during progression into G2 phase. Increased expression of various cyclins have been noted in some human cancers.
  • Tested applicationsIHC-P, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) (N terminal).

  • Positive control
    • Tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab16726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/40.
WB Use at an assay dependent dilution. 1/100 - 1/500.
IP Use at an assay dependent dilution. 1/100 - 1/500.
ICC/IF 1/1000.

Target

  • FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCN1 antibody
    • CCN1 antibody
    • CCNA antibody
    • CCNA antibody
    • CCNA2 antibody
    • CCNA2_HUMAN antibody
    • Cyclin A2 antibody
    • Cyclin A2 antibody
    • Cyclin A2 antibody
    • Cyclin-A antibody
    • Cyclin-A2 antibody
    see all

Anti-Cyclin A2 antibody [6E6] images

  • ICC/IF image of ab16726 stained HeLa cells (ab150035). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16726, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) ab150113) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab16726 staining human tonsil by IHC-P.

References for Anti-Cyclin A2 antibody [6E6] (ab16726)

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (u2os)
Specification u2os
Permeabilization Yes - 0.5% triton
Fixative Paraformaldehyde
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Submitted Jan 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Human Cell (TIG-1 primary fibroblasts)
Specification TIG-1 primary fibroblasts
Permeabilization Yes - 0.2% Triton-X100
Fixative Paraformaldehyde
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Verified customer

Submitted Sep 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"