Overview

  • Product nameAnti-Cyclin A2 antibody
    See all Cyclin A2 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin A2
  • SpecificityReacts with cyclin A2.
  • Tested applicationsICC/IF, IHC-Fr, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human

    Does not react with

    African Green Monkey
  • Immunogen

    Synthetic peptide:

    HSKYHSVSLLNPPETLSV

    , corresponding to C terminal amino acids 405-422 of Mouse Cyclin A.

  • Positive control
    • K562, HeLa IF/ICC: HeLa cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab7956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 20133761Use at an assay dependent dilution (PMID 20133761).
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 49 kDa.Can be blocked with Cyclin A peptide (ab8011).
IHC-P 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Overnight incubation may increase the signal with this antibody (see Abreview).

Target

Anti-Cyclin A2 antibody images

  • ab7956 at 1/150 staining developing mouse embryo tissue sections (5 microns) by IHC-P. The tissue was formaldehyde fixed and and heat mediated antigen retrieval step in citric acid buffer was performed. The tissue was blocked in BSA and incubated with the antibody for 2 hours. A biotinylated goat antibody was used as the secondary. The image shows T/S of developing oesophagus, trachea, skeletal muscle and blood vessel wall.

    See Abreview

  • ICC/IF image of ab7956 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7956, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Cyclin A2 antibody (ab7956)

This product has been referenced in:
  • Li Y  et al. Acute myocardial infarction induced functional cardiomyocytes to re-enter the cell cycle. Am J Transl Res 5:327-35 (2013). WB ; Rat . Read more (PubMed: 23634243) »
  • Li YG  et al. Polysaccharide from Phellinus linteus induces S-phase arrest in HepG2 cells by decreasing calreticulin expression and activating the P27kip1-cyclin A/D1/E-CDK2 pathway. J Ethnopharmacol 150:187-95 (2013). WB . Read more (PubMed: 24001891) »

See all 19 Publications for this product

Product Wall

Thank you for your reply.

I am sorry about the issues you have been having with ab7956 in western blot. As requested I am sending you ab38 as a free of charge replacement for you and you should have that tomorrow morning. For your records you...

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Thank you for your reply.

Based on the information that you have provided, I think the best way forward is to replace the antibody for you. Since we do not have a different lot that we can send you as a replacement, I have placed the links to ...

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Thank you for contacting Abcam.

The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P & IHC-Fr on mouse samples. To be able to help you further in this matter would you be able to let me know how the...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample African Green Monkey Cell lysate - whole cell (CV-1)
Total protein in input 500 µg
Specification CV-1
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

Submitted May 21 2012


Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab39 und ab7956 in der grünen Meerkatze zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Si...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde bisher noch keiner unsererAnti-Cyclin A Produktein der Westliche Grünmeerkatze (Chlorocebus sabaeus) getestet. Eine BLAST Analy...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (developing embryo)
Specification developing embryo
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid 10mM pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jun 26 2007

The peptide sequence is MOUSE: HSKYHSVSLLNPPETLSV also known as cyclin A2.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"