Overview

  • Product nameAnti-Cyclin A2 antibody [E23.1]See all Cyclin A2 primary antibodies ...
  • Description
    Mouse monoclonal [E23.1] to Cyclin A2
  • Tested applicationsIHC-P, WB, IP, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Fusion protein corresponding to Cow Cyclin A.

  • Positive control
    • In Western Blot, ab38 gavre a positive signal in the following whole cell lysates: HEK293; U20S; PC12; NIH3T3. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab38 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa).
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 22802651

Target

Anti-Cyclin A2 antibody [E23.1] images

  • All lanes : Anti-Cyclin A2 antibody [E23.1] (ab38) at 5 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes


  • Predicted band size : 60 kDa

    Julian Gannon, CRUK.

    Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD. Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD.
  • Immunoprecipitation using ab38 at 4µg/mg whole cell lysate diluted in RIPA buffer and incubated with Protein G matrix at 4°C for 12 hours.

    This antibody can immunoprecipitate Cyclin A from both Human (293T) and Rat (PC12) cell extract. The species assigned as Cyclin A runs at  ~55 kDa.

    Lanes 1-3 = 293T

    Lanes 4-6 = PC12

    Lane 1: Cyclin A antibody IP

    Lane 2: Control antibody IP

    Lane 3: 293T whole cell lysate at 10%

    Lane 4: Cyclin A antibody IP

    Lane 5: Control antibody IP

    Lane 6: PC12 whole cell lysate at 10%

    The Western Blot antibody was ab38 at 2µg/ml

    See Abreview

  • All lanes : Anti-Cyclin A2 antibody [E23.1] (ab38) at 2 µg/ml

    Lane 1 : U2OS (Hu) whole cell lysate
    Lane 2 : 293T (Hu) whole cell lysate
    Lane 3 : Cos-7 (AGMk) whole cell lysate
    Lane 4 : CHO (Hm) whole cell lysate
    Lane 5 : PC12 (Rat) whole cell lysate
    Lane 6 : 3T3 (Ms) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP conjugated goat anti-mouse at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 6 minutes

    This image is courtesy of an anonymous Abreview

    The membrane was blocked with 5% milk for 1 hour at 21°C prior to incubation with the primary antibody for 12 hours at 4°C.

    See Abreview

  • IHC image of Cyclin A staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Cyclin A2 antibody [E23.1] (ab38)

This product has been referenced in:
  • Sharon E  et al. Human herpesvirus 6 (HHV-6) alters E2F1/Rb pathways and utilizes the E2F1 transcription factor to express viral genes. Proc Natl Acad Sci U S A 111:451-6 (2014). Human . Read more (PubMed: 24335704) »
  • Hagen KR  et al. Silencing CDK4 radiosensitizes breast cancer cells by promoting apoptosis. Cell Div 8:10 (2013). WB ; Human . Read more (PubMed: 23886499) »

See all 12 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample Human Cell (Osteosarcoma)
Specification Osteosarcoma
Permeabilization Yes - triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 15 2014



The non hazardous SDS forms have been placed as 'SDS' PDF files available for download from the online product datasheets for ab9574 and ab87352 on Abcam's website.

Alternatively I have placed links to the PDF files below:

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (corneal endothelium)
Specification corneal endothelium
Fixative Formaldehyde
Permeabilization Yes - 1% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Dr. Zhiguo He

Verified customer

Submitted Nov 06 2012

Application Immunocytochemistry
Sample Human Cultured Cells (corneal endothelial cells in vitro)
Specification corneal endothelial cells in vitro
Fixative Paraformaldehyde
Permeabilization Yes - 1% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Dr. Zhiguo He

Verified customer

Submitted Nov 06 2012

Thank you for contacting us.

Your credit note ID is XXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in on...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital ...

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Thank you very much for confirming the details.

We are unsure about the identity of bands. Not many complaints are received for this antibody infact this is the second since December 2011 so protocol troubleshooting might help.

There ...

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Thanks you very much for your email. I am very sorry to hear that you are experiencing problems with this antibody.

I have checked the image and details you have kindly provided and in order to know more about the problem I have following ques...

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (293T)
Total protein in input 200 µg
Specification 293T
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Nov 13 2007

Application Western blot
Sample Human Cell lysate - whole cell (U2OS and 293T cell line)
Loading amount 30 µg
Specification U2OS and 293T cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Nov 07 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"