Overview

  • Product name
    Anti-Cyclin A2 antibody [E67.1]
    See all Cyclin A2 primary antibodies
  • Description
    Mouse monoclonal [E67.1] to Cyclin A2
  • Tested applications
    Suitable for: ICC/IF, WB, ICC, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, African green monkey
  • Immunogen

    Full length protein (Cow).

  • Epitope
    The epitope for this antibody was mapped by phage selection - selected phage contained the sequence LDYP. This would place the epitope within the region spanning amino acids 144 - 148 of human Cyclin A2. Cyclin As that contain the epitope Cow A2 Human A2 Hamster A2 Mouse A2 Cyclin As that do not contain the epitope Chicken A2 Frog A2 Human A1 Mouse A1 Frog A1
  • General notes
    Few other monoclonal antibodies other than E67.1 are able to immunoprecipitate active CDK/Cyclin A H1 kinase from such a wide range of species.

Properties

Applications

Our Abpromise guarantee covers the use of ab39 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 18077453
WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
ICC Use at an assay dependent concentration.
IP Use a concentration of 2.5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

Images

  • Cells at stages in the cell cycle from quiescence to mitosis (1-8) were loaded onto SDSPAGE and blotted with anti CDK2 antibody. Note all cells have similar amounts of CDK2. In the lower panel these same extracts were first immunoprecipitated with anti Cyclin A monoclonal E67. In these lanes, only the CDK2 that is complexed to Cyclin A is immunoblotted. Note only the CDK2 in the later stages of the cell cycle is immunoprecipitated, which is when Cyclin A is made, This clearly shows that this antibody is able to immunoprecipitate cyclin A when it is bound to CDK2.
  • ab39 at a 1/100 dilution staining Cyclin A in African Green Monkey COS-7 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in methanol and an Alexa-Fluor 555 conjugated anti-mouse secondary was used at a 1/3000 dilution.

References

This product has been referenced in:
  • Sahara N  et al. Characteristics of TBS-extractable hyperphosphorylated tau species: aggregation intermediates in rTg4510 mouse brain. J Alzheimers Dis 33:249-63 (2013). Read more (PubMed: 22941973) »
  • Gao Z & Godbout R Serine phosphorylation regulates disabled-1 early isoform turnover independently of Reelin. Cell Signal 23:555-65 (2011). WB . Read more (PubMed: 21111810) »

See all 4 Publications for this product

Customer reviews and Q&As

Vielen Dank für Ihren Anruf.

Meine Kollegin von derBestellabteilunghat Ihren Fall an mich weitergeleitet.

Wir haben Ihre Bestellung mit dem abgelaufenen Discountcode für ab39 durchgehen lassen, ich moechte Sie aber bitten, i...

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Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an, da wir bei über 92.000 Produkten gar nicht die logistischen Möglichkeiten haben, neben unserer eigentlichen Produktpalette Teströhrchen zu lagern.
Da nach Ihrer Aussage di...

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Ich denke wirklich, dass Ihr Problem mit diesen Aks nicht auf den WB begrenzt ist , da mir die ICC Färbung nicht spezifisch erscheint. Ich würde die Färbung definitiv eher nuklear lokalisiert erwarten, und das ist sie einfach nicht.
Wir verwenden ...

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Ich habe Ihnen einen Blot mit dem ab32498 an diese Email gehängt: von einem Schmier keine Spur! Was mir allerdings aufgefallen ist, ist dass der Verdünnungsfaktor um 10 verkleinert wurde. Ich habe unser Datenblatt dementsprechend verändert. Falls ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
African Green Monkey Cell lysate - whole cell (CV-1)
Total protein in input
500 µg
Specification
CV-1
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted May 21 2012


Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab39 und ab7956 in der grünen Meerkatze zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Si...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde bisher noch keiner unsererAnti-Cyclin A Produktein der Westliche Grünmeerkatze (Chlorocebus sabaeus) getestet. Eine BLAST Analy...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
African Green Monkey Cultured Cells (COS-7)
Specification
COS-7
Fixative
Methanol
Permeabilization
Yes - Methanol
Username

Abcam user community

Verified customer

Submitted Mar 31 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Fibroblast)
Specification
Fibroblast
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 for 10mins
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 14 2008

I'm very sorry to hear you are having problems with ab38. We do not have a recommended starting dilution so I would like to recommend trying a higher concentration of antibody (try 10ug/ml). We have only received one complaint regarding this popula...

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1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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