Overview

  • Product nameAnti-Cyclin A2 antibody [Y193]
    See all Cyclin A2 primary antibodies
  • Description
    Rabbit monoclonal [Y193] to Cyclin A2
  • Tested applicationsIHC-P, ICC, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cyclin A2 aa 400-500 (C terminal).

  • Positive control
    • WB: HeLa cell lysate. IHC-P: Skin carcinoma.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC
IP
WB
ICC/IF
  • Application notesICC: 1/100 - 1/250.
    IHC-P: 1/50 - 1/100.
    IP: 1/50.
    WB: 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

    Is unsuitable for FACS.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCN1 antibody
      • CCNA antibody
      • CCNA2 antibody
      • CCNA2_HUMAN antibody
      • Cyclin A2 antibody
      • Cyclin-A antibody
      • Cyclin-A2 antibody
      see all

    Anti-Cyclin A2 antibody [Y193] images

    • Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/1000 dilution + HeLa cell lysate

      Predicted band size : 48 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    • Ab32386, at a 1/50 dilution, staining Cyclin A in paraffin embedded skin carcinoma tissue sections by Immunohistochemistry.Ab32386, at a 1/50 dilution, staining Cyclin A in paraffin embedded skin carcinoma tissue sections by Immunohistochemistry.

    References for Anti-Cyclin A2 antibody [Y193] (ab32386)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization No
    Fixative Methanol
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Aug 20 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"