Recombinant
RabMAb

Anti-Cyclin A2 antibody [Y193] (ab32386)

Overview

  • Product name
    Anti-Cyclin A2 antibody [Y193]
    See all Cyclin A2 primary antibodies
  • Description
    Rabbit monoclonal [Y193] to Cyclin A2
  • Tested applications
    Suitable for: IHC-P, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cyclin A2 aa 400 to the C-terminus (C terminal).

  • Positive control
    • WB: HeLa, HepG2, HEK293, NIH/3T3 cell lysates. IHC-P: Skin and colon carcinoma. ICC/IF: MCF7 cells. IP: HeLa cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Cyclin A2 antibody (Alexa Fluor® 568) [Y193] (ab210980)

    Anti-Cyclin A2 antibody (Alexa Fluor® 594) [Y193] (ab211581)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab32386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500.
IP 1/40 - 1/50.
WB 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

For unpurified, use 1/1000.

ICC/IF 1/100 - 1/250.

Target

  • Function
    Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
  • Sequence similarities
    Belongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stage
    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCN1 antibody
    • CCNA antibody
    • Ccna2 antibody
    • CCNA2_HUMAN antibody
    • Cyclin A2 antibody
    • Cyclin-A antibody
    • Cyclin-A2 antibody
    see all

Images

  • Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/10000 dilution (purified) + NIH/3T3 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/10000 dilution (purified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : HEK293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • ab32386 (purified) at 1/40 immunoprecipitating Cyclin A2 in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 55 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • Immunofluorescence staining of MCF7 cells with purified ab32386 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4 % PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32386 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemical staining of paraffin embedded human colon carcinoma with purified ab32386 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/1000 dilution (unpurified) + HeLa cell lysate

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Coleman KE  et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017). Read more (PubMed: 28475037) »
  • Chen S  et al. Long non-coding RNA PTENP1 inhibits proliferation and migration of breast cancer cells via AKT and MAPK signaling pathways. Oncol Lett 14:4659-4662 (2017). WB ; Human . Read more (PubMed: 29085464) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast cancer cell line xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Breast cancer cell line xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 23 2015

Application
Immunocytochemistry
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Oct 26 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% acrylamide)
Loading amount
50 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mrs. Vanessa Maillet

Verified customer

Submitted Aug 27 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
No
Fixative
Methanol
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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