Overview

  • Product name
  • Description
    Rabbit polyclonal to Cyclin B1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Sandwich ELISA, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Cyclin B1 (C terminal).
    Database link: P14635

  • Positive control
    • Jurkat, Ls174T or HeLa cells. Breast carcinoma tissue section.

Properties

Applications

Our Abpromise guarantee covers the use of ab2949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 0.2 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.2 µg/ml with Mouse monoclonal [7A9] to Cyclin B1 (ab49215) as Capture.
IHC-P Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at 10 µg/mg of lysate.
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).

Target

Images

  • Standard Curve for Cyclin B1 (Analyte: Cyclin B1 protein (His tag) (ab40591)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [7A9] to Cyclin B1 (ab49215) at 0.2µg/ml and Detector Antibody Rabbit polyclonal to Cyclin B1 (ab2949) at 0.2µg/ml.
  • Breast carcinoma stained with ab2949
  • ab2949 staining Cyclin B1 in mouse MEF cells by Immunocytochemistry/ immunoflurescence. Cells were fixed with paraformaldehyde and blocking with 2% serum was performed for 2 hours at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA, 0.3% Triton X-100 in PBS) for 18 hours at 4°C. An Alexa Fluor®594-conjugated goat polyclonal to rabbit IgG was used diluted 1/1000 as secondary antibody. Red is cyclinB1 staining, blue is DAPI staining DNA for nuclei, green is vinculin staining.

    See Abreview

References

This product has been referenced in:
  • Burger K & Gullerova M Nuclear re-localization of Dicer in primary mouse embryonic fibroblast nuclei following DNA damage. PLoS Genet 14:e1007151 (2018). Read more (PubMed: 29394246) »
  • Meng X  et al. MicroRNA-125b-1-3p mediates intervertebral disc degeneration in rats by targeting teashirt zinc finger homeobox 3. Exp Ther Med 15:2627-2633 (2018). Read more (PubMed: 29456666) »

See all 9 Publications for this product

Customer reviews and Q&As

Filter by Application

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Application
Western blot
Sample
Human Cell lysate - whole cell (Human Cell lysate - whole cell (Epithelial OVCAR c)
Gel Running Conditions
Reduced Denaturing (10 % SDS-PAGE)
Loading amount
100 µg
Treatment
Perifosine 0, 12, 24 hr
Specification
Human Cell lysate - whole cell (Epithelial OVCAR c
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Apr 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Specification
MEF
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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