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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab7957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr IHC-Fr: Use at an assay dependent dilution.
IP IP: Use at an assay dependent dilution.
WB WB: Use at an assay dependent dilution. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).Can be blocked with Cyclin B1 (C-term) peptide (human) (ab8012).

Target

  • FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Post-translational
    modifications    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.

    • Target information above from: UniProt accession P14635 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        CCNB 1 antibodyCCNB antibodyCCNB1 antibody
        CCNB1_HUMAN antibodyG2 mitotic specific cyclin B1 antibodyG2/mitotic-specific cyclin-B1 antibody
      see all

    Anti-Cyclin B1 antibody images

    • Western blot - Cyclin B1 antibody (ab7957)
      Western blot - Cyclin B1 antibody (ab7957)


      Predicted band size : 49 kDa


      ab7957 staining Cyclin B1 in HeLa whole cell lysate

    References for Anti-Cyclin B1 antibody (ab7957)

    This product has been referenced in:

    See all 3 Publications for this product

    Publishing research using ab7957 ? Please let us know so that we can cite the reference in this datasheet

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    Western blot abreview for Anti-Cyclin B1 antibody

     Excellent
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa cells)
    Loading amount 100000 cells
    Specification HeLa cells
    Treatment Released from a double thymidine block
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step 2% milk and 0.5% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C
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    Verified customer

    Submitted Sep 07 2009

    Please could you tell me which part of the synthetic peptide (human) was used to generate anti-cyclin B1 ab7957. I am interested to know if this antibody has been generated to recognise the N terminal of cyclin B1, the C terminal, or full length cy...

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    ab7957 is made with a peptide mapping to amino acids 414-433 of human origin (this is the carboxy terminus).

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"