Anti-Cyclin B1 antibody [CCNB1/1098] (ab212977)
Key features and details
- Mouse monoclonal [CCNB1/1098] to Cyclin B1
- Suitable for: IHC-P, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cyclin B1 antibody [CCNB1/1098]
See all Cyclin B1 primary antibodies -
Description
Mouse monoclonal [CCNB1/1098] to Cyclin B1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chinese hamster -
Immunogen
Recombinant full length protein corresponding to Human Cyclin B1 aa 1 to the C-terminus.
Database link: P14635 -
Positive control
- IHC: Human tonsil, testicular carcinoma, endometrial carcinoma, prostate carcinoma and ovarian carcinoma tissues. ICC: MCF-7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab212977 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
CCNB1/1098 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT). |
|
ICC |
Use a concentration of 5 µg/ml.
|
Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). |
ICC
Use a concentration of 5 µg/ml. |
Target
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Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 100770842 Chinese hamster
- Entrez Gene: 327679 Cow
- Entrez Gene: 891 Human
- Entrez Gene: 268697 Mouse
- Entrez Gene: 25203 Rat
- Omim: 123836 Human
- SwissProt: Q08301 Chinese hamster
- SwissProt: Q1LZG6 Cow
see all -
Alternative names
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
Images
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Immunofluorescence Analysis of formaldehyde-fixed MCF-7 cells stained with 5 ug/mL ab212977 (red) and DNA is stained with Hoechst (blue).
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Immunofluorescence Analysis of formaldehyde-fixed MCF-7 cells stained with 5 ug/mL ab212977 (red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Cyclin B1 with ab212977 at 1 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testicular carcinoma tissue labeling Cyclin B1 with ab212977 at 1 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human endometrial carcinoma tissue labeling Cyclin B1 with ab212977 at 1 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling Cyclin B1 with ab212977 at 1 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling Cyclin B1 with ab212977 at 1 µg/ml.
Datasheets and documents
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Datasheet download
References (1)
ab212977 has been referenced in 1 publication.
- Lai MC et al. Experimental and clinical evidence suggests that GRPEL2 plays an oncogenic role in HCC development. Am J Cancer Res 11:4175-4198 (2021). PubMed: 34659882