Overview

  • Product name
    Anti-Cyclin B1 antibody [V92.1]
    See all Cyclin B1 primary antibodies
  • Description
    Mouse monoclonal [V92.1] to Cyclin B1
  • Specificity
    V92.1 can immunoprecipitate active CDK1/cyclin B1 complexes.
  • Tested applications
    Suitable for: ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein (Hamster).

Properties

Applications

Our Abpromise guarantee covers the use of ab18221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IP
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Essential for the control of the cell cycle at the G2/M (mitosis) transition.
    • Sequence similarities
      Belongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stage
      Accumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Post-translational
      modifications
      Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
    • Cellular localization
      Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCNB 1 antibody
      • CCNB antibody
      • ccnb1 antibody
      • CCNB1_HUMAN antibody
      • Cyclin B1 antibody
      • G2 mitotic specific cyclin B1 antibody
      • G2/mitotic-specific cyclin-B1 antibody
      see all

    Images

    • ICC/IF image of ab18221 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18221, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    This product has been referenced in:
    • Garner AP  et al. Delta MEKK3:ER* activation induces a p38 alpha/beta 2-dependent cell cycle arrest at the G2 checkpoint. Oncogene 21:8089-104 (2002). Read more (PubMed: 12444545) »
    • Minemoto Y  et al. Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53. Exp Cell Res 262:37-48 (2001). Read more (PubMed: 11120603) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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