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Mouse monoclonal [V92.1] to Cyclin B1
V92.1 can immunoprecipitate active CDK1/cyclin B1 complexes.
Full length protein (Hamster).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Belongs to the cyclin family. Cyclin AB subfamily.
Accumulates steadily during G2 and is abruptly destroyed at mitosis.
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
Information by UniProt
CCNB 1 antibody
Immunocytochemistry/ Immunofluorescence - Cyclin B1 antibody [V92.1] (ab18221)
ICC/IF image of ab18221 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18221, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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