Validated using a knockout cell line
Recombinant
RabMAb

Anti-Cyclin B1 antibody [Y106] - BSA and Azide free (ab156447)

Overview

  • Product name
    Anti-Cyclin B1 antibody [Y106] - BSA and Azide free
    See all Cyclin B1 primary antibodies
  • Description
    Rabbit monoclonal [Y106] to Cyclin B1 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cyt, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cyclin B1 aa 400-500 (C terminal). The exact sequence is proprietary.

  • General notes

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.

Target

  • Function
    Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • Sequence similarities
    Belongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stage
    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNB 1 antibody
    • CCNB antibody
    • ccnb1 antibody
    • CCNB1_HUMAN antibody
    • Cyclin B1 antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

References

ab156447 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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