Overview

  • Product name
    Anti-Cyclin B1 (phospho S126) antibody
    See all Cyclin B1 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin B1 (phospho S126)
  • Specificity
    Minimal reactivity is expected with the non-phosphorylated form of the protein.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin B1 aa 120-131 (phospho S126).
    Sequence: ILVDTASpPSPME
    Database link: P14635

  • General notes


    This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.

Properties

Applications

Our Abpromise guarantee covers the use of ab3488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/100.

Target

Images

  • All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : H23 whole cell lysate
    Lane 3 : Hepatocarcinoma (Hep3b) cell lysate
    Lane 4 : Glioblastoma (T98G) cell lysate
    Lane 5 : B cell lymphoblast (Daudi) cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa


    Exposure time : 20 seconds

References

This product has been referenced in:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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