For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Full length protein (Xenopus laevis).
Our Abpromise guarantee covers the use of ab18250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 45 kDa. The antibody may give a high background if the concentration of the antibody is above 2µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
This image is courtesy of an anonymous Abreview