Overview

  • Product nameAnti-Cyclin B3 antibody
    See all Cyclin B3 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin B3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    a region within synthetic peptide: MLLPLPPQSS KPVPKKSQSS KIVPSHHDPS EKTGENCQTK ISPSSLQESP, corresponding to N terminal amino acids 1-50 of Human Cyclin B3

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab66759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 158 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceCyclin B3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event.This cyclin may associate with CDC2 and CDK2 kinases, and be required for proper spindle reorganization and restoration of the interphase nucleus.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • CCNB 3 antibody
    • CYCB3 antibody
    • G2/mitotic specific cyclin B3 antibody

Anti-Cyclin B3 antibody images

  • Anti-Cyclin B3 antibody (ab66759) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 158 kDa
    Observed band size : 12 kDa (why is the actual band size different from the predicted?)

References for Anti-Cyclin B3 antibody (ab66759)

ab66759 has not yet been referenced specifically in any publications.

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