Anti-Cyclin C antibody (ab85927)
Key features and details
- Rabbit polyclonal to Cyclin C
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Cyclin C antibody -
Description
Rabbit polyclonal to Cyclin C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide corresponding to Human Cyclin C aa 233-283.
Database link: P24863 -
Positive control
- Panc1, 293T, Jurkat, H2009 and HeLa whole cell lysate (ab150035).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
|
IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. -
Tissue specificity
Highest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain. -
Sequence similarities
Belongs to the cyclin family. Cyclin C subfamily.
Contains 1 cyclin N-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 421791 Chicken
- Entrez Gene: 509717 Cow
- Entrez Gene: 892 Human
- Entrez Gene: 51813 Mouse
- Entrez Gene: 100153639 Pig
- Entrez Gene: 114839 Rat
- Omim: 123838 Human
- SwissProt: P55168 Chicken
see all -
Alternative names
- ccnc antibody
- CCNC_HUMAN antibody
- CycC antibody
see all
Images
-
All lanes : Anti-Cyclin C antibody (ab85927) at 0.1 µg/ml
Lane 1 : Panc1 whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : H2009 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Cyclin C with ab85927 at 1/1000 (1µg/ml). Detection: DAB.
-
Detection of Human Cyclin C in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85927 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab85927 has been referenced in 6 publications.
- Haarhuis JHI et al. A Mediator-cohesin axis controls heterochromatin domain formation. Nat Commun 13:754 (2022). PubMed: 35136067
- Li M et al. Emodin regulates cell cycle of non-small lung cancer (NSCLC) cells through hyaluronan synthase 2 (HA2)-HA-CD44/receptor for hyaluronic acid-mediated motility (RHAMM) interaction-dependent signaling pathway. Cancer Cell Int 21:19 (2021). PubMed: 33407495
- Liu L et al. An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness. Nat Commun 12:2693 (2021). PubMed: 33976158
- Lu L et al. Overexpression of miR-15b Promotes Resistance to Sunitinib in Renal Cell Carcinoma. J Cancer 10:3389-3396 (2019). PubMed: 31293642
- Xiaoli AM et al. Lipogenic SREBP-1a/c transcription factors activate expression of the iron regulator hepcidin, revealing cross-talk between lipid and iron metabolisms. J Biol Chem 294:12743-12753 (2019). PubMed: 31270208
- Fukuda A et al. Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene. PLoS One 8:e72496 (2013). PubMed: 23967313