Overview

  • Product name
  • Description
    Rabbit polyclonal to Cyclin C
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residues 244 and 294 of Human Cyclin C (NP_005181.2).

  • Positive control
    • Panc1, 293T, Jurkat, H2009 and HeLa whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab85927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.
  • Tissue specificity
    Highest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain.
  • Sequence similarities
    Belongs to the cyclin family. Cyclin C subfamily.
    Contains 1 cyclin N-terminal domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ccnc antibody
    • CCNC_HUMAN antibody
    • CycC antibody
    • Cyclin C antibody
    • Cyclin-C antibody
    • hSRB11 antibody
    • OTTHUMP00000016897 antibody
    • SRB11 homolog antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Cyclin C with ab85927 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Cyclin C antibody (ab85927) at 0.1 µg/ml

    Lane 1 : Panc1 whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : H2009 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 33 kDa
    Observed band size : 33 kDa


    Exposure time : 3 minutes
  • Detection of Human Cyclin C in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85927 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

This product has been referenced in:
  • Fukuda A  et al. Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene. PLoS One 8:e72496 (2013). Read more (PubMed: 23967313) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us. Unfortunately, ab85927 has not been tested for cross-reactivity with CDK8 or CDK3. Based on the UniProt sequences, the immunogen region for this antibody shows no significant similarity to CDK3 or CDK8 and we would not expe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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