Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin D1 antibody [EPR2241]

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Human Cell (MCF-7)
Specification MCF-7
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% goat serum+0.1% BSA+0.1% Triton+PBS
Dilution 1/100

Secondary antibody

Name Non-Abcam antibody was used: Alexa Fluor 488 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution 1/500

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Submitted Aug 04 2014