Overview

  • Product name
  • Description
    Rabbit polyclonal to Cyclin D2
  • Specificity
    No cross reactivity with other proteins.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminal of Human Cyclin D2.

Properties

Applications

Our Abpromise guarantee covers the use of ab81359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.

Target

  • Function
    Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • Sequence similarities
    Belongs to the cyclin family. Cyclin D subfamily.
    Contains 1 cyclin N-terminal domain.
  • Cellular localization
    Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCND 2 antibody
    • ccnd2 antibody
    • CCND2_HUMAN antibody
    • CyclinD2 antibody
    • G1/S specific cyclin D2 antibody
    • G1/S-specific cyclin-D2 antibody
    • KIAK0002 antibody
    • MGC102758 antibody
    • MPPH3 antibody
    see all

Images

  • All lanes : Anti-Cyclin D2 antibody (ab81359) at 1 µg/ml

    Lane 1 : Tissue lysates prepared from Rat testicle tissue.
    Lane 2 : Tissue lysates prepared from Rat ovary tissue.
    Lane 3 : Tissue lysates prepared from Rat placental tissue.
    Lane 4 : Whole cell lysates prepared from MCF7 cells.
    Lane 5 : Whole cell lysates prepared from MM231 cells.
    Lane 6 : Whole cell lysates prepared from Hela cells.
    Lane 7 : Whole cell lysates prepared from HT1080 cells.
    Lane 8 : Whole cell lysates prepared from colo320 cells.

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

    Predicted band size : 33 kDa

References

ab81359 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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