Overview

  • Product nameAnti-Cyclin D2 antibody
    See all Cyclin D2 primary antibodies
  • Description
    Mouse polyclonal to Cyclin D2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Cyclin D2 (NP_001750.1)

  • Positive control
    • Human placenta tissue lysate. A-431 cell lysate. Cyclin D2 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab89595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.

Target

  • FunctionRegulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin D subfamily.
    Contains 1 cyclin N-terminal domain.
  • Cellular localizationNucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCND 2 antibody
    • ccnd2 antibody
    • CCND2_HUMAN antibody
    • CyclinD2 antibody
    • G1/S specific cyclin D2 antibody
    • G1/S-specific cyclin-D2 antibody
    • KIAK0002 antibody
    • MGC102758 antibody
    see all

Anti-Cyclin D2 antibody images

  • Anti-Cyclin D2 antibody (ab89595) at 1 µg/ml + Human placenta tissue lysate at 50 µg

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.
  • Anti-Cyclin D2 antibody (ab89595) at 1 µg/ml + A-431 cell lysate at 50 µg

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-Cyclin D2 antibody (ab89595) at 1 µg/ml

    Lane 1 : Cyclin D2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 33 kDa

References for Anti-Cyclin D2 antibody (ab89595)

ab89595 has not yet been referenced specifically in any publications.

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