• Product nameAnti-Cyclin D3 antibody [EP463E]
    See all Cyclin D3 primary antibodies
  • Description
    Rabbit monoclonal [EP463E] to Cyclin D3
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cyclin D3 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control
    • K562 cell lysate; HeLa cells.
  • General notes

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.


Associated products


Our Abpromise guarantee covers the use of ab52598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
Flow Cyt 1/30. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionRegulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin D subfamily.
      Contains 1 cyclin N-terminal domain.
    • Cellular localizationNucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCND 3 antibody
      • Ccnd3 antibody
      • CCND3_HUMAN antibody
      • CyclinD3 antibody
      • D3 type cyclin antibody
      • G1 S specific cyclin D3 antibody
      • G1/S specific cyclin D3 antibody
      • G1/S-specific cyclin-D3 antibody
      see all

    Anti-Cyclin D3 antibody [EP463E] images

    • Anti-Cyclin D3 antibody [EP463E] (ab52598) at 1/5000 dilution + K562 cell lysate at 10 µg

      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
    • Immunofluorescent staining of HeLa cells using ab52598 at a dilution of 1/100-1/250.
    • ab2582 staining human MDA-MB-436 breast cancer cells by Flow Cytometery. Cells were treated with Trypsin/EDTA for 5 minutes, sample buffer prepared as 5%FCS in PBS and gating was done on live non apoptotic cells. The primary antibody diluted 1/200 and incubated with sample for 1 hour at 4°C. A undiluted FITC conjugated goat polyclonal to rabbit IgG was used as secondary.

      See Abreview

    References for Anti-Cyclin D3 antibody [EP463E] (ab52598)

    This product has been referenced in:
    • Misiewicz-Krzeminska I  et al. Post-transcriptional Modifications Contribute to the Upregulation of Cyclin D2 in Multiple Myeloma. Clin Cancer Res 22:207-17 (2016). Read more (PubMed: 26341922) »
    • Brodowska K  et al. Effects of metformin on retinoblastoma growth in vitro and in vivo. Int J Oncol 45:2311-24 (2014). Read more (PubMed: 25215935) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Hep G2)
    Gel Running Conditions Reduced Denaturing
    Loading amount 20 µg
    Specification Hep G2
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Mar 20 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (MDA-MB-436 breast cancer cells)
    Specification MDA-MB-436 breast cancer cells
    Preparation Cell harvesting/tissue preparation method: Trypsin/EDTA for 5 minutes
    Sample buffer: 5% FCS in PBS
    Permeabilization No
    Gating Strategy live (non apoptotic cells) were selected

    Dr. Penelope Ottewell

    Verified customer

    Submitted Dec 16 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (sub-cutaneous MDA-MB-436 breast tumour)
    Specification sub-cutaneous MDA-MB-436 breast tumour
    Treatment 2 mg/kg doxorubicin and 0.1 mg/kg zoledronic acid
    Gel Running Conditions Reduced Non-Denaturing (Native) (10 % polyacrylamide gel)
    Blocking step (agent) for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

    Dr. Penelope Ottewell

    Verified customer

    Submitted Jun 27 2008