Anti-Cyclin D3 antibody [SPM241], prediluted (ab54238)

Overview

  • Product nameAnti-Cyclin D3 antibody [SPM241], prediluted
    See all Cyclin D3 primary antibodies
  • Description
    Mouse monoclonal [SPM241] to Cyclin D3, prediluted
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Monkey
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • LS174T cells. Human breast carcinoma or tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab54238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: Ready to use for 30 min at room temperature.
    Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRegulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin D subfamily.
      Contains 1 cyclin N-terminal domain.
    • Cellular localizationNucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCND 3 antibody
      • Ccnd3 antibody
      • CCND3_HUMAN antibody
      • CyclinD3 antibody
      • D3 type cyclin antibody
      • G1 S specific cyclin D3 antibody
      • G1/S specific cyclin D3 antibody
      • G1/S-specific cyclin-D3 antibody
      see all

    Anti-Cyclin D3 antibody [SPM241], prediluted images

    • Ab54238, prediluted, staining Cyclin D3 in formalin fixed, paraffin embedded human tonsil tissue by immunohistochemistry.

    References for Anti-Cyclin D3 antibody [SPM241], prediluted (ab54238)

    ab54238 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab54238.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"