Anti-Cyclin D3 (phospho T283) antibody (ab182639)

Overview

  • Product name
    Anti-Cyclin D3 (phospho T283) antibody
    See all Cyclin D3 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin D3 (phospho T283)
  • Host species
    Rabbit
  • Specificity
    ab182639 detects endogenous levels of Cyclin D3 only when phosphorylated at Threonine 283.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin D3 (phospho T283) conjugated to Keyhole Limpet Haemocyanin (KLH). (T-S-T(p)-P-T).
    Database link: P30281

  • Positive control
    • Mouse heart tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab182639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 33 kDa.

Target

  • Function
    Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • Sequence similarities
    Belongs to the cyclin family. Cyclin D subfamily.
    Contains 1 cyclin N-terminal domain.
  • Cellular localization
    Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCND 3 antibody
    • Ccnd3 antibody
    • CCND3_HUMAN antibody
    • CyclinD3 antibody
    • D3 type cyclin antibody
    • G1 S specific cyclin D3 antibody
    • G1/S specific cyclin D3 antibody
    • G1/S-specific cyclin-D3 antibody
    see all

Images

  • All lanes : Anti-Cyclin D3 (phospho T283) antibody (ab182639) at 1/500 dilution

    Lane 1 : Mouse heart tissue lysate with immunizing peptide
    Lane 2 : Mouse heart tissue lysate

    Predicted band size: 33 kDa

References

ab182639 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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