Overview

  • Product nameAnti-Cyclin E1 antibody
    See all Cyclin E1 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin E1
  • SpecificityDetects endogenous levels of total Cyclin E1 protein.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin E1.

  • Positive control
    • Human colon carcinoma tissue, extracts from HeLa cells treated with Paclitasel.

Properties

Applications

Our Abpromise guarantee covers the use of ab52189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
  • Tissue specificityHighly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin E subfamily.
  • Post-translational
    modifications
    Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 antibody images

  • All lanes : Anti-Cyclin E1 antibody (ab52189) at 1/500 dilution

    Lane 1 : extracts from HeLa cells, treated with Paclitasel (1uM, 60mins)
    Lane 2 : extracts from HeLa cells, treated with Paclitasel (1uM, 60mins) with blocking peptide


    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • Ab52189 at 1/50 dilution staining Human colon carcinoma, with and without blocking peptide; paraffin embedded.Ab52189 at 1/50 dilution staining Human colon carcinoma, with and without blocking peptide; paraffin embedded.

References for Anti-Cyclin E1 antibody (ab52189)

This product has been referenced in:
  • Chao C  et al. Epstein-Barr virus infection and expression of B-cell oncogenic markers in HIV-related diffuse large B-cell Lymphoma. Clin Cancer Res 18:4702-12 (2012). Read more (PubMed: 22711707) »
  • Ramathal C  et al. Lack of CCAAT enhancer binding protein beta (C/EBPbeta) in uterine epithelial cells impairs estrogen-induced DNA replication, induces DNA damage response pathways, and promotes apoptosis. Mol Cell Biol 30:1607-19 (2010). IHC-P ; Mouse . Read more (PubMed: 20086097) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"