The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
Tissue specificityHighly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Sequence similaritiesBelongs to the cyclin family. Cyclin E subfamily.
Post-translational modificationsPhosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
All lanes : Anti-Cyclin E1 antibody (ab52189) at 1/500 dilution
Lane 1 : extracts from HeLa cells, treated with Paclitasel (1uM, 60mins) Lane 2 : extracts from HeLa cells, treated with Paclitasel (1uM, 60mins) with blocking peptide
Predicted band size : 47 kDa Observed band size : 47 kDa
Anti-Cyclin E1 antibody (ab52189)
Ab52189 at 1/50 dilution staining Human colon carcinoma, with and without blocking peptide; paraffin embedded.Ab52189 at 1/50 dilution staining Human colon carcinoma, with and without blocking peptide; paraffin embedded.
References for Anti-Cyclin E1 antibody (ab52189)
This product has been referenced in:
Chao C et al. Epstein-Barr virus infection and expression of B-cell oncogenic markers in HIV-related diffuse large B-cell Lymphoma. Clin Cancer Res18:4702-12 (2012).
Read more (PubMed: 22711707) »
Ramathal C et al. Lack of CCAAT enhancer binding protein beta (C/EBPbeta) in uterine epithelial cells impairs estrogen-induced DNA replication, induces DNA damage response pathways, and promotes apoptosis. Mol Cell Biol30:1607-19 (2010).
Read more (PubMed: 20086097) »