Overview

  • Product nameAnti-Cyclin E1 antibody
    See all Cyclin E1 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin E1
  • Specificityab52239 detects endogenous levels of total Cyclin E1 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin E1. Immunogen range is 91-140

  • Positive control
    • K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab52239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 47 kDa).
ELISA 1/20000.

Target

  • FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
  • Tissue specificityHighly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin E subfamily.
  • Post-translational
    modifications
    Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 antibody images

  • All lanes : Anti-Cyclin E1 antibody (ab52239) at 1/300 dilution

    Lane 1 : K562 cell extract; without peptide
    Lane 2 : K562 cell extract; with peptide


    Predicted band size : 47 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

References for Anti-Cyclin E1 antibody (ab52239)

ab52239 has not yet been referenced specifically in any publications.

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