• Product nameAnti-Cyclin E1 antibody
    See all Cyclin E1 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin E1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Cyclin E1.

  • Positive control
    • HepG2 or K562 nuclear lysate Mouse or rat liver nuclear lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.



Our Abpromise guarantee covers the use of ab71535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
  • Tissue specificityHighly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin E subfamily.
  • Post-translational
    Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 antibody images

  • IHC image of Cyclin E staining in Human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71535, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Cyclin E1 antibody (ab71535) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Rat liver lysate

    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References for Anti-Cyclin E1 antibody (ab71535)

This product has been referenced in:
  • Serna A  et al. Functional inhibition of aquaporin-3 with a gold-based compound induces blockage of cell proliferation. J Cell Physiol 229:1787-801 (2014). WB ; Rat . Read more (PubMed: 24676973) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rabbit Cell (skeletal myoblasts)
Permeabilization Yes - triton x-100
Specification skeletal myoblasts
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted May 11 2017

Application Western blot
Sample Human Cell lysate - whole cell (Human Epithelial OVCAR cell line)
Gel Running Conditions Reduced Denaturing (10 % SDS-PAGE)
Loading amount 100 µg
Treatment Perifosine 0-24 hr
Specification Human Epithelial OVCAR cell line
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Apr 20 2017

Thank you for contacting Abcam.

The actual predicted molecular weight of Cyclin E is 47kDa, but when we tested it we were detecting bands in the 55kDa region. I do not think that there is anything for you to be concerned about as it could just...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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